Thesis
ANALYSIS OF VIABILITY, VARIABILITY AND PRESENCE OF ANTIMICROBIAL RESISTANCE GENES IN EQUINE PROBIOTICS
Washington State University
Master of Science (MS), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000003152
Handle:
https://hdl.handle.net/2376/122301
Abstract
Probiotics, by definition, are live microorganisms which when consumed in adequate amounts confer a health benefit to the host. The objective of this thesis is to critically evaluate some definition criteria of a probiotic and commercial probiotic label claims, highlighting areas for improvement in probiotic development. The first study evaluates the effect of an in vitro simulation of the equine proximal GI tract on probiotic viability. It suggests that enteric protection should be considered to improve microorganism viability in equine probiotics and that increasing gastric pH may improve survival of some microorganisms. The second study evaluated the content and inter-lot variability of commercial equine probiotic products using culture dependent and independent techniques. This study found that none of the evaluated commercial equine probiotic samples met their label claim for quantity and type of viable microorganisms but only two of eleven demonstrated inter-lot variability. In the third study, the objective is to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed for use in animals that claimed to contain Enterococcus spp. It identified vanA in 2 out of 36 commercially available probiotics marketed for use in animals that also contained Enterococcus spp. DNA. This thesis highlights areas for improvement in probiotic development.
Metrics
8 File views/ downloads
34 Record Views
Details
- Title
- ANALYSIS OF VIABILITY, VARIABILITY AND PRESENCE OF ANTIMICROBIAL RESISTANCE GENES IN EQUINE PROBIOTICS
- Creators
- Ana Claudia Ramos Berreta
- Contributors
- Macarena Sanz (Advisor)Jamie J Kopper (Committee Member)Craig McConnel (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 79
- Identifiers
- 99900652003401842
- Language
- English
- Resource Type
- Thesis