Thesis
Acoustic manipulation of C. elegans in microfluidic devices
Washington State University
Master of Science (MS), Washington State University
2012
Handle:
https://hdl.handle.net/2376/102593
Abstract
The ability to precisely trap, transport and manipulate micrometer-sized objects is very important in microfluidic applications. Caenorhabditis elegans (C. elegans) is one of the most ubiquitous lab animals used for biological research; and for most experimental studies and observations it needs to be selected, transported or immobilized. In this study we demonstrate the use of acoustic radiation forces, including Primary Radiation Force (PRF) and Secondary Radiation Force (SRF), for manipulating C. elegans. PRF in a standing wave field is used to trap multiple C. elegans. Simulations are found to be in good agreement with the patterns of the trapped C. elegans. SRF generated around an array of insonated microbubbles is used to concentrate C. elegans and precisely manipulate a single C. elegans to direct its motion in a specified path. Theoretical explanations for using both PRF and SRF to trap and manipulate worms are presented. Due to their non-contact and non-electric-field nature, we believe that these inexpensive methodologies can pave their way for novel lab on a chip technologies for manipulating C. elegans, as well as potentially any other important biological samples.
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Details
- Title
- Acoustic manipulation of C. elegans in microfluidic devices
- Creators
- Gan Yu
- Contributors
- Jie Xu (Degree Supervisor)Xiaolin Chen (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Electrical Engineering and Computer Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525143001842
- Language
- English
- Resource Type
- Thesis