Thesis
Alternative polyadenilation events in the uterus in dairy cattle diagnosed with endometritis under certified organic management
Washington State University
Master of Science (MS), Washington State University
2018
Handle:
https://hdl.handle.net/2376/105183
Abstract
Endometritis (inflammation of the endometrial lining) is one of the most important reproductive diseases, resulting in more than $650 million in lost revenue annually in US dairy farms. Here we hypothesize that alternative polyadenylation (APA) sites serve as decisive sensors for endometrium health and disease. Endometrial cells collected from 18 cows with purulent vaginal discharge scored 0 (clinically healthy), 1 (<50% of purulent material) or 2 (>50% of purulent material) were used for APA site profiling with our newly developed method. Overall, pathogens trigger hosts to use more differentially expressed APA (DE-APA) sites,, more intronic DE-APA sites, more DE-APA sites per gene and more DE-genes (DEGs) associated with inflammation. Host CD59 (CD59 molecule), FCGR2A (Fc fragment of IgG receptor IIa), LY75 (lymphocyte antigen 75) and PLG (plasminogen) may serve as initial contacts or combats with pathogens on cell surface, followed by activation of NR1H4 (nuclear receptor subfamily 1 group H member 4) to regulate AXL (AXL receptor tyrosine kinase), FGR (FGR proto-oncogene, Src family tyrosine kinase), HCK (HCK proto-oncogene, Src family tyrosine kinase) and ITGB2 (integrin subunit beta 2) for anti-inflammation. This study is the first to show significance of cilium pathways in endometrium health. MIR21 and MIR30A would be ideal antagonistic biomarkers for diagnosis of either inflammation or anti-inflammation. These novel findings will set precedent for future genomic studies to aid the dairy industry develop new strategies to reduce endometritis incidence and improve fertility.
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Details
- Title
- Alternative polyadenilation events in the uterus in dairy cattle diagnosed with endometritis under certified organic management
- Creators
- Meagan Jolene Stotts
- Contributors
- Martin G. Maquívar (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Animal Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525300601842
- Language
- English
- Resource Type
- Thesis