Thesis
An evaluation of hypernodulating pea (Pisum sativum L.) lines for biological nitrogen fixation in a wheat (Triticum aestivum)-pea intercropping system
Washington State University
Master of Science (MS), Washington State University
2011
Handle:
https://hdl.handle.net/2376/102338
Abstract
Three factorial experiments were executed under greenhouse conditions with pea lines differeing at genes controlling a dwarf and hypernodulation phenotypes in a common genetic background to test the effectiveness and efficiency of nitrogen fixation under different nitrogen fertility conditions and in an intercropping system with Louise , a spring wheat variety. The experiments were conducted concurrently under similar conditions with the same genotypes. Genotypic effects were observed on several measures of biomass, nodule number, percent nitrogen, total nitrogen in shoots and seeds. Fertility effects were observed in % Ndfa in shoots and seeds of wheat. Genotype X fertility interactions were not observed in most these traits.There was no significant difference between Rondo and the hypenodulating isoline Rondonod3 for % N or total N in shoots under intercropping, but both lines differed from the Rondonod3-dwf line. The Rondo-nod3-dwf had the highest % N in shoots but the least amount of total nitrogen due to its reduced biomass. The dwarf line had lower %N and total N in the seeds than the other lines. The Rondo-nod3 line had slightly less % N in seeds than Rondo but had similar amounts of total N it its seeds. There were significant differences in wheat performance caused by the intercropping treatments on shoot weight, seed weight, and percent nitrogen in seed. Wheat grown as a sole crop had the highest above-ground biomass compared to wheat grown under intercropping with different pea genotypes. There was variability on pea-wheat treatment combinations on biomass produced by wheat. Significant differences in wheat seed yield were also observed due to the different intercropped pea treatments; whether the peas were removed or killed after seven weeks or allowed to mature. There were genotypic differences in shoot weight, root nodule number, percent nitrogen and total nitrogen in the shoots, roots and C: N ratio in monoculture and in intercropping. However, no significant genotype differences were observed in percent nitrogen between Rondo-nod3 and Rondo-nod3-dwf lines when intercropped with wheat. The Rondo has a potential role in improving the soil fertility in a monocrop, green manure or as a cover crop.
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Details
- Title
- An evaluation of hypernodulating pea (Pisum sativum L.) lines for biological nitrogen fixation in a wheat (Triticum aestivum)-pea intercropping system
- Creators
- Thumbiko Walker Gambler Mkandawire
- Contributors
- Scot H. Hulbert (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Crop and Soil Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900524807401842
- Language
- English
- Resource Type
- Thesis