Thesis
Analysis of Protein and Tannin Content in Vinifera Wine using Spectrophotometric and Turbidimetric Methods
Washington State University
Master of Science (MS), Washington State University
2023
DOI:
https://doi.org/10.7273/000005334
Abstract
Understanding the complex interactions between proteins and tannins in wines is crucial for assessing wine quality and stability. In this study, we investigated the protein-tannin interactions in Syrah wines using a combination of spectrophotometric and turbidimetric methods. To determine the protein content, we employed the bicinchonic acid assay (BCA), a widely used colorimetric method. Various proteins were used as internal standards for comparative analysis. Additionally, we utilized a turbidimeter alongside a dose-response curve to monitor the formation of haze in a model wine matrix environment, which served as an indicator of protein-tannin interactions across the same suite of proteins. Our results revealed significant interactions between proteins and tannins in Syrah wines, meditated by pH and ethanol content. The BCA assay indicated variations in protein content among the wines tested. Moreover, the turbidimetric analysis demonstrated varying degrees of haze formation, reflecting the diverse protein-tannin complex formations. Our study provides valuable insights into the protein-tannin interactions in Syrah wines and highlights the importance of protein as a factor in red wine quality and stability.
Metrics
2 File views/ downloads
26 Record Views
Details
- Title
- Analysis of Protein and Tannin Content in Vinifera Wine using Spectrophotometric and Turbidimetric Methods
- Creators
- Ryan Doyle
- Contributors
- James F. Harbertson (Advisor)Thomas Collins (Committee Member)Thomas Henick-Kling (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Food Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 94
- Identifiers
- 99901031339801842
- Language
- English
- Resource Type
- Thesis