Thesis
Analysis of reactive oxygen species generation during post-harvest physiological deterioration of cassava (Manihot esculenta crantz) storage roots
Washington State University
Master of Science (MS), Washington State University
2012
Handle:
https://hdl.handle.net/2376/101450
Abstract
Cassava (Manihot esculenta Crantz) is a major food source in several African and South American countries with two significant drawbacks: high concentrations of cyanogenic glycosides and the rapid degradation of tubers. Using fluorescent microscopy, analysis of reactive oxygen species (ROS), hydrogen peroxide, superoxide and hydroxyl radical, localization and concentration demonstrated the connection between cassava post-harvest physiological degradation (PPD) and ROS. A three-day time course post-harvest confirmed mitochondrial produced ROS is an integral part of PPD development as well as reporting other sources of ROS generation. In addition, while hydrogen peroxide fluorescence increased, the variability between each time point indicates that several other mechanisms, such as scopoletin peroxidase and other pathogen defense processes interact with the initial wounding response to generate the series of events recognized as PPD development. Finally, due to the difficulty of studying short-lived molecules, this first analysis of superoxide and hydroxyl radical concentrations presented a previously undetected peak of superoxide 24 hours after harvest, while hydroxyl radical concentrations are far lower than predicted. In conclusion this study demonstrated not only the importance of the first day after harvest but also the presented the first evidence of cytosolic superoxide generation within Cassava storage tubers.
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Details
- Title
- Analysis of reactive oxygen species generation during post-harvest physiological deterioration of cassava (Manihot esculenta crantz) storage roots
- Creators
- Erin Nicole Getzin
- Contributors
- John K. Fellman (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Program in Molecular Plant Sciences
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525375801842
- Language
- English
- Resource Type
- Thesis