Thesis
Antibiotic Resistance of Bacillus cereus and its Survival, Growth, and Toxin Production During Cooking and Storage of Fresh Rice Noodles
Washington State University
Master of Science (MS), Washington State University
2023
DOI:
https://doi.org/10.7273/000005336
Abstract
This research studied the survival, growth, and toxin production of B. cereus in fresh rice noodles (FRNs) and the antibiotic resistance of B. cereus in spacecraft assembly facilities. B. cereus, a spore-forming and foodborne pathogenic bacterium widely distributed in the environment and food sources, leads to both health concerns for consumers and spoilage challenges for food producers. In the food sector, this pathogen causes food illnesses and food spoilage because it can resist harsh conditions and survive most processing steps. In the space industry, where microbial contamination and monitoring keep crews healthy, this pathogen can resist regular cleaning programs. The FRNs study aimed to find ideal storage conditions to preserve the quality and increase the safety of FRNs. The antibiotic resistance study sought to understand the profile of antibiotic resistance in B. cereus to help the space industry monitor bacterial infections during spacecraft flight. The findings of the FRNs study showed that B. cereus survives steam cooking, grows, and produces enterotoxin during storage at 22 and 32°C. At these temperatures, a decrease in pH, a sign of spoilage in FRNs, was also observed. However, refrigeration temperature (4°C) did not promote the growth and toxin production of B. cereus during storage. A significant interaction effect of storage time and temperature was observed in growth, toxin production, and pH changes (p<0.05). The antibiotic resistance study found that B. cereus is resistant to β-lactam antibiotics, sulfa/trimeth, and rifampin. However, all B. cereus isolates showed susceptibility to gentamicin, 97% to clindamycin, and 95% to chloramphenicol, imipenem, tetracycline, and vancomycin. The findings of the FRNs study should assist food producers and retail stores to ensure the safety of their products. Similarly, the antibiotic resistance study should help the space industry to improve the treatment programs for bacterial infections during space flight.
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Details
- Title
- Antibiotic Resistance of Bacillus cereus and its Survival, Growth, and Toxin Production During Cooking and Storage of Fresh Rice Noodles
- Creators
- Barakatullah Mohammadi
- Contributors
- Stephanie Smith (Advisor)Carolyn Ross (Committee Member)Meijun Zhu (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Food Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 109
- Identifiers
- 99901031340201842
- Language
- English
- Resource Type
- Thesis