Thesis
Application of digitally-driven mass spectrometry to ultra high mass biomolecules
Washington State University
Master of Science (MS), Washington State University
2015
Handle:
https://hdl.handle.net/2376/102923
Abstract
The development of matrix-assisted laser desorption ionization (MALDI) and electrosprary ionization (ESI) permitted scientists to use mass spectrometers to analyze biomolecules. However, the range of biomolecular masses was greater than the working range of the mass spectrometers. Consequently, large biomolecules have to be systematically fragmented or multiply charged so that their mass-to-charge ratio fits within the working range of the mass spectrometer. The work describe herein evaluates the capability of digitally operated quadrupole time-of-flight (Q-TOF) mass spectrometry for comprehensive analysis with ultra high mass biomolecules with ultra high resolution and mass accuracy. Our newly developed inlet, trapping system, and digital waveform technology permits trapped ions to be injected into the TOF in a well-collimated ion beam to obtain high resolution and mass accuracy at any protein mass. Rapid quantitation of complex protein distribution is now feasible by mass spectrometry. Application of the digitally operated Q-TOF mass spectrometer was extended in this work by developing general procedures for sample analysis\\ and data interpretion. The major findings from this work include: 1) controlling the charge on intact proteins by ESI charge reduction; 2) high resolution TOF mass analysis of the entire range.
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Details
- Title
- Application of digitally-driven mass spectrometry to ultra high mass biomolecules
- Creators
- Huijuan Chen
- Contributors
- Peter T. A. Reilly (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemistry, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525170501842
- Language
- English
- Resource Type
- Thesis