Thesis
Atomic force microscopy characterization of normal and cancer prostate cells
Washington State University
Master of Science (MS), Washington State University
2010
Handle:
https://hdl.handle.net/2376/102672
Abstract
The cell mechanical properties in terms of the parameters adhesion, Young's, and dilation modulus and stiffness values for the prostate cell lines RWPE-1 (benign), RWPE-2 (nonmetastatic), DU145 (moderately metastatic from brain metastasis), and PC3 (highly metastatic from bone metastasis) have been determined by atomic force microscopy (AFM). A large number of force-distance approach curves were obtained to discern differences between the cell lines. Significant differences in all the parameters were observed between the cell lines as indicated by statistical analysis of the data. In general it was found that as cells transitioned from a normal to a highly metastatic phenotype they had higher elasticity and stiffness, and greater ability to dilate and bend. The densities of cell surface molecules and average interaction distances of these molecules and AFM cantilever tip were also determined. A long and short interaction distance with the cantilever tip at small and large indentations was observed. These interaction distances increased from normal to highly metastatic cells with a decrease in the corresponding molecular densities. Taken together these results indicate that the response of normal and cancer cells to nanoindentation forces are significantly different reflecting their phenotypes.
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Details
- Title
- Atomic force microscopy characterization of normal and cancer prostate cells
- Creators
- Chandima Bandara Bandaranayaka Mudiyanselage
- Contributors
- Subra Muralidharan (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Molecular Biosciences, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525378801842
- Language
- English
- Resource Type
- Thesis