Thesis
Biogenic marker detection for high-throughput phenotyping of Aphanomyces root rot resistance in pea
Washington State University
Master of Science (MS), Washington State University
2018
Handle:
https://hdl.handle.net/2376/101550
Abstract
The biogenic emissions from plants are related to complex plant traits such as growth, development, and their defense mechanisms against biotic and abiotic stress. In this study, our overall goal was to identify biogenic markers associated with resistance to Aphanomyces root rot in pea. Aphanomyces root rot, caused by Aphanomyces euteiches Drechs. f. sp. pisi, is a prominent disease in peas (Pisum sativum L.). The disease symptoms include disintegrated roots, and chlorosis and necrosis of plant foliage. In this work, gas chromatography-flame ionization detector (GC-FID) with gas chromatography-mass spectrometry (GC-MS) and pyrolysis gas chromatography-mass spectrometry (Py-GC-MS) were used to identify biogenic markers associated with potential disease resistance in pea. Two pea cultivars, cv 'Ariel' (susceptible) and cv 'Hampton' (tolerant) were inoculated with A. euteiches, and biogenic plant volatiles collected from healthy (control) pea plants were compared to inoculated pea plants every five days from five days post inoculation until flowering using GC-MS and GC-FID. The data collection was performed using dynamic headspace sampling technique with solid-phase microextraction (SPME) fiber. Finally, we conducted Py-GC-MS analysis for detecting the biogenic chemical marker present within the roots and shoots of the pea plants. Immediately after volatile analysis, roots and shoots of the plants were placed in liquid nitrogen and stored at 20oC until Py-GC-MS analysis. The samples were pyrolyzed at 500oC and the chemical compounds present in the sample were analyzed. In volatile analysis, three major compounds were identified: (Z)-3-hexenyl acetate, (Z)-hexen-1-ol, and hexanal. In addition, Py-GC-MS analysis indicated presence of the phenolic compounds 2-methoxy-4-venyl phenol, 3-methoxy phenol, and stigmasterol, that are associated with plant defense mechanisms. The overall findings indicate that biogenic biomarkers can serve as a non-destructive high-throughput phenotyping technique to determine disease resistance in pea.
Metrics
21 File views/ downloads
45 Record Views
Details
- Title
- Biogenic marker detection for high-throughput phenotyping of Aphanomyces root rot resistance in pea
- Creators
- Abirami Rajendran
- Contributors
- Sindhuja Sankaran (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Systems Engineering, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525124501842
- Language
- English
- Resource Type
- Thesis