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CHARACTERIZATION OF PUTATIVE FATTY ACID DESATURASES IN MELOIDOGYNE INCOGNITA AND MELOIDOGYNE JAVANICA1.05 MB
CC BY-NC V4.0, Open Access
Thesis
CHARACTERIZATION OF PUTATIVE FATTY ACID DESATURASES IN MELOIDOGYNE INCOGNITA AND MELOIDOGYNE JAVANICA
Washington State University
Master of Science (MS), Washington State University
01/2022
DOI:
https://doi.org/10.7273/000004543
Handle:
https://hdl.handle.net/2376/125137
Abstract
Fatty acids are fundamental molecular building blocks of all living organisms. They are essential for a slew of the basic processes of cellular life such as energy storage, membrane integrity and fluidity, and mitigating oxidative stress. They also play an important role in whole organism development and behavior as precursors to essential signaling molecules. Fatty acids make up the majority of nematode energy stores, and a reduction in fatty acid metabolism has shown detrimental effects on growth, behavior, and reproduction in the nematode as demonstrated in the model organism C. elegans. Studies in parasitic root-knot nematodes (RKN) have shown similarly that a loss of lipid reserves reduces survivability and infectivity. This makes the fatty acid metabolism a target of interest for the development of new nematicides, however; very little work has been done to explore and characterize the metabolic pathways in RKN. To understand this pathway better we used RNAi to study the effect of knocking down putative fatty acid desaturases in M. incognita and M. javanica. We developed both exogenous and endogenous RNAi delivery systems to test the effects of knock-down of desaturase gene homologs within each species. One homolog was knocked down in M. javanica both in vitro and in vivo. We found that not only could this gene be successfully knocked down but doing so had a phenotypical response of reducing root galling in the host plant.
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Details
- Title
- CHARACTERIZATION OF PUTATIVE FATTY ACID DESATURASES IN MELOIDOGYNE INCOGNITA AND MELOIDOGYNE JAVANICA
- Creators
- Zoe Sehlke
- Contributors
- Jennifer L Watts (Advisor)Cynthia Gleason (Committee Member)Philip F Mixter (Committee Member)Jon M Oatley (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Molecular Biosciences, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 79
- Identifiers
- OCLC#: 1371069374; 99900898538401842
- Language
- English
- Resource Type
- Thesis