Thesis
Catecholamine innervation of the ventrolateral bed nucleus of the stria terminalis: role in corticosterone and feeding responses to glucose deficit
Washington State University
Master of Science (MS), Washington State University
12/2009
DOI:
https://doi.org/10.7273/000006459
Abstract
The ventrolateral bed nucleus of the stria terminalis (vlBNST), a nucleus that is implicated in the responses to a variety of stressors, is heavily innervated by hindbrain catecholamine neurons. The majority of these are norepinephrine (NE) neurons, although some epinephrine (E) neurons may contribute. Some of these catecholamine neurons co-innervate the paraventricular nucleus of the hypothalamus (PVH). We previously found that PVH injections of DSAP, the retrogradely-transported immunotoxin anti-dopamine beta hydroxylase (DBH) conjugated to saporin (SAP); profoundly reduced feeding, hyperglycemic and corticosterone responses to glucoprivation. However, the PVH DSAP injections did not alter satiety induced by cholecystokinin (CCK), which has been linked to catecholamine neurons in the A2 cell group. In this experiment, we examined the role of the catecholamine projection to the vlBNST in the corticosterone responses to glucoprivation and swim stress, the feeding and hyperglycemic responses to glucoprivation, and the feeding response to CCK-8.
The distribution of DBH-immunoreactive (-ir) cell bodies lost in the hindbrain after vlBNST DSAP injection was predominantly from NE, as opposed to E, cell groups. The DSAP induced loss of DBH neurons in the dorsal hindbrain was concentrated in the A2 cell group (14.6 – 13.68 mm caudal to bregma), where a maximum of 56% and an average of 48% of DBH neurons were destroyed. In ventral hindbrain, loss of DBH cell bodies was predominantly in the A1 cell group (14.6 – 12.8 mm caudal to bregma), a maximum of 64% and an average of 55% of DBH-ir neurons were destroyed. DSAP lesions did not impair the feeding or blood glucose responses to 2-deoxy-D-glucose (2DG)-induced glucoprivation and did not impair the suppression of feeding by CCK-8 (2 ug/kg), indicating that the catecholamine neurons that innervate the vlBNST, including the dually-projecting neurons that innervate both the vlBNST and the PVH, are not required for these responses. The corticosterone response to 5-min swim was not impaired by the DSAP injection, but the corticosterone response to 2DG, although still robust, was significantly attenuated, suggesting that the vlBNST may contribute to, the corticosterone response to glucoprivation
Metrics
1 File views/ downloads
13 Record Views
Details
- Title
- Catecholamine innervation of the ventrolateral bed nucleus of the stria terminalis
- Creators
- Huston Nathaneal
- Contributors
- Sue Ritter (Chair)Robert Ritter (Committee Member) - Washington State University, Department of Integrative Physiology and NeuroscienceHeiko Jansen (Committee Member) - Washington State University, Department of Integrative Physiology and Neuroscience
- Awarding Institution
- Washington State University
- Academic Unit
- Program in Neuroscience
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 42
- Identifiers
- 99901102224701842
- Language
- English
- Resource Type
- Thesis