Thesis
Characterization and analysis of an air-segmented continuous flow analyzer for small-scale mass transfer limited systems
Washington State University
Master of Science (MS), Washington State University
2000
Handle:
https://hdl.handle.net/2376/42
Abstract
An optimum reaction scheme for the use of the glucose oxidase Trinder reagent on the ASCFA was developed using mathematical modeling techniques coupled with experimental measurements. The optimized reaction involved decreasing the glucose oxidase concentration to 6.7 x 10-5 mM, by a factor of 18.8, while maintaining constant the concentrations of all other reagents. This slows the reaction so oxygen is not depleted in the 88 sec. time frame designated for detection. Theophylline analyses performed on the ASCFA proved challenging but not impossible. Hindrance to slug-flow mixing was shown to be due to the high viscosity of the theophylline RS solution. Pre-mixing of the sample and reagent, and incubation until the reaction nears completion, alleviated the mixing problem. This investigation indicates that an ASCFA module can provide a compact chemical analysis component for a POCT analyzer. It can process a variety of standard diagnostic tests in less than three minutes, while not compromising accuracy or precision. With reagents adapted specifically for the ASCFA module, even reactant-limited assays such as those for detecting glucose, using glucose oxidase Trinder reagent, or mass transferlimited assays such as that for the detection of theophylline, can be performed accurately over linear, diagnostically significant ranges.
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Details
- Title
- Characterization and analysis of an air-segmented continuous flow analyzer for small-scale mass transfer limited systems
- Creators
- Jennifer Ann Seidl
- Contributors
- Bernard J. Van Wie (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525085601842
- Language
- English
- Resource Type
- Thesis