Thesis
Characterization of PDX1.2 and a novel PDX1.2 interactor
Washington State University
Master of Science (MS), Washington State University
2018
Handle:
https://hdl.handle.net/2376/101850
Abstract
Vitamin B6 is a crucial compound in all living organisms based on its capacity to function as a cofactor in more than 140 biochemical processes. In addition the vitamin has been demonstrated to be a powerful antioxidant that prevents cell damage. In plants, the de novo pathway depends on the concerted activities of PDX1 (Pyridoxine Biosynthesis Protein 1) and PDX2 that are able to catalyze the formation of a PLP synthase. In Arabidopsis thaliana, PDX1 encodes for three homologs PDX1.1, PDX1.2, and PDX1.3, but only PDX1.1 and PDX1.3 are able to interact with PDX2. While the functions of PDX1.1 and PDX1.3 have been extensively studied, the role of PDX1.2 has only recently started to become resolved. PDX1.2 has been identified in previous studies to be up-regulated under certain abiotic stressors such as heat, oxidative, and UV-B stress. It is also been observed to stabilize the other PDX1 proteins under a heat stress. It was previously shown via size-exclusion chromatography that AtPDX1.2 assembles into a complex of around 750 kDa, which is around the size of a PLP synthase. Since PDX1.2 does not interact with PDX2, it appeared to be likely that PDX1.2 assembles with another, yet unknown protein. A yeast-2-hybrid screen found an unknown and uncharacterized protein that exclusively interacts with PDX1.2. The work overall provides a better understanding of the role PDX1.2, and its involvement in the biosynthesis of vitamin B6 and stress tolerance. It looks at a novel PDX1.2 interactor called NPI1 and its impact on development, stress tolerance, and a potential role in the biosynthesis of vitamin B6.
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Details
- Title
- Characterization of PDX1.2 and a novel PDX1.2 interactor
- Creators
- Seth Nathaniel Stahl
- Contributors
- Hanjo Hellmann (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Sciences, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525378601842
- Language
- English
- Resource Type
- Thesis