Thesis
Comparative analysis of pulmonary immune responses to Mycoplasma ovipneumoniae in domestic sheet (Ovis aries) and Bighorn sheep (Ovis canadensis)
Washington State University
Master of Science (MS), Washington State University
05/2019
DOI:
https://doi.org/10.7273/000000057
Handle:
https://hdl.handle.net/2376/118894
Abstract
Mycoplasma ovipneumoniae is a respiratory pathogen that impacts domestic sheep (Ovis
aries; DS) and bighorn sheep (Ovis canadensis; BHS). While experimental and observational
field data indicate BHS are more susceptible than DS to bacterial pneumonia, immunopathologic
correlates of this susceptibility difference in relation to M. ovipneumoniae infection remain
uncharacterized. Typical histopathologic findings in lungs of both species infected with M.
ovipneumoniae included hyperplasia of bronchiolar epithelium and bronchial associated
lymphoid tissue (BALT), and mononuclear leukocyte infiltration around bronchioles and in
adjacent alveolar walls. While similar by light microscopic evaluation, we hypothesized that a
differential interspecies immunopathologic pulmonary response to M. ovipneumoniae infection
occurs in DS and BHS. To characterize this response, a retrospective study utilizing formalinfixed, paraffin-embedded (FFPE) lung tissues from 8 uninfected and 8 infected sheep of each
species was performed. FFPE tissue was H&E stained for light microscopic evaluation and
immunohistochemically stained with antibodies specific for cytokine and leukocyte markers. Of 33 antibodies tested, 6 exhibited immunoreactivity in both species: T cell marker CD3, B cell
markers CD20 and CD79a, macrophage markers CD163 and Iba1, and cytokine IL-17.
Immunohistochemistry (IHC) stained tissue slides were scanned for colorimetric digital analysis.
Species and infection status factors were evaluated by two-way ANOVA, and Tukey’s multiple
comparison tests were performed (P<0.05 significant). CD3 and CD163 were more abundant in
infected BHS as compared to both uninfected BHS and infected DS. CD20 was more abundant
in infected DS as compared to both uninfected DS and infected BHS. CD79a was more abundant
in infected DS as compared to uninfected DS. IL-17 abundance was less in infected DS as
compared to uninfected DS. No significant differences were observed with Iba-1 abundance.
These findings suggest DS and BHS have a differential immune response to M. ovipneumoniae
infection which may in part explain the documented susceptibility difference.
Metrics
5 File views/ downloads
30 Record Views
Details
- Title
- Comparative analysis of pulmonary immune responses to Mycoplasma ovipneumoniae in domestic sheet (Ovis aries) and Bighorn sheep (Ovis canadensis)
- Creators
- PAIGE CORINNE GROSSMAN
- Contributors
- MARGARET ANN HIGHLAND (Degree Supervisor) - Washington State University, Paul G. Allen School for Global Animal HealthDONALD PATRICK KNOWLES (Committee Member) - Washington State University, Veterinary Microbiology and Pathology, Department ofDAVID SCHNEIDER (Committee Member) - Washington State University, Veterinary Microbiology and Pathology, Department ofSANTANU BOSE (Committee Member) - Washington State University, Veterinary Microbiology and Pathology, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Format
- pdf
- Number of pages
- 32
- Identifiers
- 99900591862601842
- Language
- English
- Resource Type
- Thesis