Thesis
Cripto-1 and amphiregulin production in transformed mammary epithelial cells grown on hydroxyapatite scaffolding
Washington State University
Master of Science (MS), Washington State University
2007
Handle:
https://hdl.handle.net/2376/104636
Abstract
The leading cancer in women is breast cancer making up 26 % of new cancer cases. Early detection of this disease is commonly linked to calcifications within the breast and typically relates to aggressive forms. Advanced stages of breast cancer metastasize to bone forming secondary lesions. This is believed to be a result of breast cancer cells forming bone-like proteins responsible for attachment and calcification typical in osteoblast cells. In addition, transformed mammary epithelial cells overproduce cripto-1 (CR) and amphiregulin (AR) proteins, which causes the cells to become more aggressive. Three cell lines representing nontransformed cells, aggressive cancerous cells, and less aggressive cancerous cells were used to identify possible correlations between CR, AR and bone-like calcifications. These cells were grown on hydroxyapatite scaffolding (a major component of bone) or plastic and studied for rate of attachment, proliferation, and production of CR and AR. It is shown here that CR increases secretion and overall production when grown on hydroxyapatite scaffolding. Also, the molecular weight of the protein identified as CR was much heavier compared to the known molecular weights. The rate of attachment was not altered when the cells were grown on hydroxyapatite but the growth of all three cell lines was delayed. Although AR did not have any significant differences it still provides a possible role in mammary tumorogenesis. In addition these results provide insight to the relationship of hydroxyapatite and CR production and their role in transformation of mammary epithelial cells.
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Details
- Title
- Cripto-1 and amphiregulin production in transformed mammary epithelial cells grown on hydroxyapatite scaffolding
- Creators
- Brena Baze Holman
- Contributors
- Gary H. Thorgaard (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Sciences, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525296501842
- Language
- English
- Resource Type
- Thesis