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Thesis
DEVELOPMENT AND EVALUATION OF A PCV-DEPENDENT VISUAL HEMOLYSIS COLOR SCALE FOR PACKED RBC BLOOD PRODUCT
Washington State University
Master of Science (MS), Washington State University
07/2024
DOI:
https://doi.org/10.7273/000007057
Abstract
Objective: To develop a PCV-dependent hemolysis color scale and to evaluate its accuracy in predicting supernatant hemoglobin concentration in packed red blood cell (pRBC) blood products for determining transfusion safety.
Design: Prospective experimental study
Setting: University veterinary teaching hospital
Interventions: A range of hemolyzed supernatant samples were made by performing serial dilutions of a pRBC blood unit. A computerized color was allocated to each hemolyzed supernatant sample by Adobe Illustrator, constructing a color scale. Study participants were then asked to estimate the hemoglobin concentration of a provided supernatant sample by using the color scale. The color estimation data was also analyzed by the authors, regarding whether a
correct determination to transfuse the blood would have been made as pRBC should not be transfused if the hemolysis is > 1%.
Measurements and Main Results: Visual inspection with the color scale was evaluated with seven supernatant test samples containing different free hemoglobin concentrations (0.3g/L~8.0g/L). The percentage of overall correct color estimation was 61.9%. The percentage of overall correct transfusion decisions to transfuse the blood product would have been 93.7%. All incorrect estimations were one color range (approximately 1.0g/L difference between the ranges) off from the correct estimation for all samples.
Conclusions: The color scale aided visual assessment of hemolysis. However, visual inspection with the color scale can still be inaccurate near the corresponding cut off color for <1% hemolysis in each PCV range. If the supernatant color of the unit is estimated to be one color range off from the cut-off color, the cell free hemoglobin concentration of the unit should be measured, and percent hemolysis should be calculated to determine if it meets the <1% hemolysis standard.
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Details
- Title
- DEVELOPMENT AND EVALUATION OF A PCV-DEPENDENT VISUAL HEMOLYSIS COLOR SCALE FOR PACKED RBC BLOOD PRODUCT
- Creators
- Hyein Jung
- Contributors
- Linda Martin (Chair)Jane Wardrop (Committee Member)Jillian Haines (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 30
- Identifiers
- 99901152438201842
- Language
- English
- Resource Type
- Thesis