Thesis
DOG CYP1A2: RELATIONSHIP BETWEEN GENE COPY NUMBER VARIATION AND PROTEIN CONTENT
Washington State University
Master of Science (MS), Washington State University
07/2024
DOI:
https://doi.org/10.7273/000007044
Abstract
Cytochrome P450 enzyme 1A2 (CYP1A2) plays a primary role in the biotransformation of numerous drugs. However, genetic variations could significantly affect therapeutic outcomes and predispose certain breeds to adverse drug reactions. Recently, a study revealed that CYP1A2 would be duplicated in some breeds of dogs. This finding led them to investigate the copy number variation (CNV) using droplet digital PCR (ddPCR). However, our study analyzed the gene copy number variation (CNV) versus protein content of the CYP1A2 in a moderately large and diverse bank of canine liver microsomes by quantitative real-time PCR. The livers came from 59 healthy adult dogs, including research beagles, research hound dogs, mixed breed dogs, greyhounds, chihuahuas, and others. Validation included revealed a poor correlation between the protein content and the Delta Ct of the sample according to the Spearman correlation analysis (Rs –0. 137; P 0.301); this was also confirmed by the ANOVA on ranks, (P=0.743), suggesting that gene copy number may not be the only determinant of the ability to metabolize drugs. These insights underscore the complexity of genetic factors that influence drug response and highlight the importance of further research in this field to guide the development of individualized medicine guidelines in dogs.
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Details
- Title
- DOG CYP1A2
- Creators
- Francisco Gonzales Zamora
- Contributors
- Tania Elena Perez Jimenez (Chair)Michael H Court (Committee Member)Nicolas F Villarino (Committee Member)Lais Malavasi (Committee Member)Martin Suarez (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 35
- Identifiers
- 99901152541001842
- Language
- English
- Resource Type
- Thesis