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CC BY V4.0, Open Access
Thesis
Determining the impact of omeprazole on the canine esophageal microbiota using a minimally invasive sampling method
Washington State University
Master of Science (MS), Washington State University
07/2024
DOI:
https://doi.org/10.7273/000007049
Abstract
Background – The impact of omeprazole on human esophageal microbiome and its associated side effects have been recently researched. While the esophageal string test (EST), a non-invasive technique, is established for sampling the human esophageal microbiome, its application in assessing the effect of omeprazole on canine esophageal microbiome has not been explored.
Objective – To assess the changes and subsequent recovery of canine esophageal microbiome following omeprazole treatment, utilizing the esophageal string test in awake dogs.
Design – Prospective longitudinal study.
Setting – University Veterinary Teaching Hospital.
Animals – 10 healthy, hospital staff-owned dogs
Methods – A prospective longitudinal design was employed, where esophageal samples were initially collected using the esophageal string test (day 0), involving oral administration of an esophageal string test (EST) capsule and subsequent retrieval after 15 minutes for pH-based segment identification. The dogs were then administered 1mg/kg omeprazole orally, twice daily for 14 days. Follow-up EST samplings were conducted on days 14 and 45. Samples were sequenced targeting the V3-V4 region of the 16Ss rRNA gene (Zymo Research Corporation®, Irvine, CA 92614) and diversity analysis was performed using alpha and beta diversity indices along with differential sequencing (DEseq) analysis.
Results- All dogs tolerated the EST capsule without adverse effects. Diversity analysis revealed no significant alterations in alpha and beta diversity (Bray-Curtis) across the time points.
Conclusions –Omeprazole therapy did not significantly alter the canine esophageal microbiome in this study. The application of EST in dogs highlights its use as a minimally invasive tool for investigating the role of the microbiome in esophageal health and disease.
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Details
- Title
- Determining the impact of omeprazole on the canine esophageal microbiota using a minimally invasive sampling method
- Creators
- Aditi Handa
- Contributors
- Jillian M. Haines (Chair)Yoko M. Ambrosini (Committee Member)Sarah C. Guess (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 31
- Identifiers
- 99901152215701842
- Language
- English
- Resource Type
- Thesis