Thesis
Developing molecular markers for a Hessian fly resistance gene in wheat & studying the effects of the wheat bread making gene (WBM) on soft white wheat quality
Washington State University
Master of Science (MS), Washington State University
12/2020
DOI:
https://doi.org/10.7273/000004179
Handle:
https://hdl.handle.net/2376/125324
Abstract
Wheat (Triticum aestivum L.) is one of the most important agricultural commodity crops in Washington. Each year, farmers face various production challenges, including Hessian fly [Mayetiola destructor (Say)], which can lead to devastating crop losses. Resistance genes are the most successful methods to manage and control this insect. Although there are at least 37 known Hessian fly resistance genes (HFR genes), few are deployed in the region, mostly due to a lack of molecular tools to support breeding efforts. Seahawk, a popular soft white spring wheat, contains resistance, but the gene identity is unknown. Using two recombinant inbred populations made from crossing soft white spring wheat cultivar Seahawk to spring club wheat cultivars JD and Melba, we set out to identify the source of resistance in Seahawk and to develop molecular markers that can be used to select for the gene in breeding populations. After acquiring the genetic marker information using Illumina's 90K SNP technology, we were able to map the resistance gene to the distal end of the short arm of chromosome 6B and created three kompetitive allele specific PCR (KASP) markers that are effective in selecting for this gene in diverse backgrounds. Utilizing the Seahawk/JD population, we also studied how the wheat bread-making locus (wbm) affects the quality of soft white and club wheat. Soft white wheat and club wheat are produced and milled for their weak gluten properties and high starch contents that result in delicate crumb structures when baked. The wbm gene, however, is known to be associated with increased gluten strength properties and was identified in elite bread-making cultivars. At Washington State, the wbm gene is found at higher than expected frequencies among elite club and soft white wheat varieties. After testing the quality parameters of wbm isolines, we found that the locus does not negatively impact soft white wheat flour or dough properties.
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Details
- Title
- Developing molecular markers for a Hessian fly resistance gene in wheat & studying the effects of the wheat bread making gene (WBM) on soft white wheat quality
- Creators
- Tavin Marie Schneider
- Contributors
- MICHAEL O. PUMPHREY (Advisor) - Washington State University, Crop and Soil Sciences, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Crop and Soil Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900896441001842
- Language
- English
- Resource Type
- Thesis