Thesis
Development and improvement of on-site molecular detection of Spongospora subterranea f. sp. subterranea and other soilborne pathogens of potato
Washington State University
Master of Science (MS), Washington State University
05/2018
DOI:
https://doi.org/10.7273/000004231
Handle:
https://hdl.handle.net/2376/125025
Abstract
Soilborne diseases are particularly difficult to diagnose because the soil environment is extremely large, relative to plant mass, and complex, making it a challenge to understand all the aspects of soilborne diseases. Moreover, soilborne diseases can be symptomless during early infection stages, dependent on environmental stressors, and some have long latent periods that result in delayed diagnoses. Presently in many diagnostic labs, polymerase chain reaction (PCR), particularly real-time PCR, is considered the most sensitive and accurate method for plant pathogen detection. On-site diagnosis of plant diseases can enable timely decisions allowing earlier implementation of disease management strategies reducing the impact of the disease. In the current study, Spongospora subterranea f. sp. subterranea (S. subterranea), Rhizoctonia solani AG-3 and Potato mop-top virus (PMTV) are used to demonstrate the potential for on-site molecular detection using a variety of methods such as portable real-time PCR, loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA). The results suggest that the presented on-site real-time PCR method is suitable for soil-based detection of S. subterranea, Rhizoctonia solani AG-3 and PMTV (PMTV). Moreover, the following results exhibit the use of RPA in detection of S. subterranea and PMTV and its potential in soil-based detection.
Metrics
6 File views/ downloads
24 Record Views
Details
- Title
- Development and improvement of on-site molecular detection of Spongospora subterranea f. sp. subterranea and other soilborne pathogens of potato
- Creators
- Joseph Breslin DeShields
- Contributors
- Kiwamu Tanaka (Advisor) - Washington State University, Plant Pathology, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Plant Pathology, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900896424401842
- Language
- English
- Resource Type
- Thesis