Thesis
EXPLORING GENETIC INSIGHTS FOR COLD TOLERANCE IN BITTER VETCH (VICIA ERVILIA (L.) WILLD.) AND ITS PROPOSED BENEFITS FOR SUSTAINABLE AGRICULTURE
Washington State University
Master of Science (MS), Washington State University
12/2024
DOI:
https://doi.org/10.7273/000007241
Abstract
Climatic shifts are increasingly transforming agricultural systems, intensifying abiotic stressors such as drought, chilling, and freezing, which adversely affect crop yields. Developing crops with resilience to these stressors is essential for maintaining global food security. Bitter vetch (Vicia ervilia (L.) Willd.), an ancient grain legume native to arid regions of the Middle East (Modern day Turkey, Syria, Israel, Iran) presents potential for agricultural diversification due to its grain and forage uses, as well as its inherent resistance to cold, drought and heat. In this study, 114 autumn-sown bitter vetch accessions of diverse origins were evaluated over three years under field conditions to assess their genetic response to cold tolerance. Cold damage scores were recorded for each accession, and a genome-wide association study employing genotype-by-sequencing data was performed to identify single nucleotide polymorphisms associated with cold tolerance. A significant SNP was identified on linkage group 4, explaining 31.31% of phenotypic
variance for cold damage. This SNP corresponds to homologous cold tolerance genes in the related common vetch (Vicia sativa) reference genome, including CPK4, PUB13, PUB24, GAD2, and SHN1, which are implicated in various cold stress responses. As a legume, bitter vetch establishes a symbiotic relationship with soil-borne rhizobia, enhancing nitrogen fixation and potentially reducing reliance on synthetic fertilizers. The findings of this research support the development of cold-tolerant bitter vetch accessions as a resilient source of nutritious grain, hay, and green manure, contributing to sustainable agricultural practices. This study addresses the challenges associated with the integration of bitter vetch into modern agricultural systems, underscoring its potential role in enhancing food security under changing climatic conditions.
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Details
- Title
- EXPLORING GENETIC INSIGHTS FOR COLD TOLERANCE IN BITTER VETCH (VICIA ERVILIA (L.) WILLD.) AND ITS PROPOSED BENEFITS FOR SUSTAINABLE AGRICULTURE
- Creators
- Britton Bourland
- Contributors
- Drew Lyon (Chair)Clarice Coyne (Committee Member)Rebecca McGee (Committee Member)Sindhuja Sankaran (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Crop and Soil Sciences
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 84
- Identifiers
- 99901195539601842
- Language
- English
- Resource Type
- Thesis