Thesis
Effect of intralesional bone marrow derived mesenchymal stem cells on collagenase induced deep digital flexor tendonitis within the digital flexor tendon sheath of horses
Washington State University
Master of Science (MS), Washington State University
2014
Handle:
https://hdl.handle.net/2376/100468
Abstract
Objective - To evaluate the effect of bone marrow derived mesenchymal stem cells (MSC)on the healing of collagenase induced deep digital flexor tendon (DDFT) injury within the digital flexor tendon sheath of horses and to document the appearance of this tendon injury as healing progressed with high field MRI. Animals - 6 horses Procedures - Horses were confirmed to be clinically sound with normal MRI of the front digits. Autologous bone marrow was collected from the tuber coxae or sternum. MSCs were cultured, expanded, and frozen at -80°C. Tendinitis of the both front DDFTs was induced with collagenase at the level of the pastern joint in each horse. One randomly assigned limb was treated with intralesional injection of MSCs 14 days post collagenase injection with the opposite limb serving as untreated control. Horses were placed into a 200 day rehabilitation program with MRI and ultrasound examinations at 0, 60, 90, and 200 days post treatment with MSCs. Horses were euthanized 200 days post treatment for tendon sample collection. Biochemical analysis of proteoglycan, DNA content, and soluble collagen was performed as well as histologic evaluation of tendon structure, cellularity, and collagen type. Results - Histologic evaluation showed significant improvements in cellular infiltration in MSC treated tendons. Biochemical analysis revealed significantly decreased amounts of glycosaminoglycan in MSC treated tendons. No difference was found between treatment groups on MRI or ultrasound. Lesion size and signal intensity increased on all sequences until 60 days post treatment then decreased until conclusion of the study on ultrasound, proton density, T2 weighted, and STIR sequences. The largest changes were seen on T2 weighted and STIR sequences. Conclusions and Clinical Relevance - Intralesional injection of MSCs improves healing of DDFT in this collagenase model of tendonitis. Treatment of horses with clinical DDFT injury may result in improved outcomes. The changes observed on MRI allow a better understanding of the progression of tendon healing and aid in determining a time table for returning horses with DDFT injury to training.
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Details
- Title
- Effect of intralesional bone marrow derived mesenchymal stem cells on collagenase induced deep digital flexor tendonitis within the digital flexor tendon sheath of horses
- Creators
- John Dylan Lutter
- Contributors
- William S. Dernell (Degree Supervisor)Julie A. Cary (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525295001842
- Language
- English
- Resource Type
- Thesis