Thesis
Effect of novel apple virus combinations on commonly used apple rootstocks and their association with apple decline in Washington state.
Washington State University
Master of Science (MS), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000003334
Handle:
https://hdl.handle.net/2376/122396
Abstract
Apple decline is an emerging disease in Washington state which is characterized by the decline and sometimes death of young trees, typically 1-2 years old. Little is known about the cause of this disease and research in this area has not produced any conclusive evidence on this troublesome problem. RNA sequencing of decline effected trees has revealed several viruses present throughout various tissues including: APMV, ASGV, ASPV, ACLSV, ARWV-2 and CCGAV. Seven different rootstocks, which are commonly used, were chosen for inoculation of different virus combinations, with four rootstocks used for the final examination due to low viral presence in the other rootstocks. 9 months after inoculation, trees were dissected so that vascular tissue could be observed for any signs of potential damage. Various tissues were also taken from these trees for relative qPCR analysis to examine viral expression of the different viruses in each rootstock used. Histological observations revealed no real vascular damage of the rootstocks looked at. qPCR analysis revealed that CCGaV has higher replication when mixed with endemic viruses. This information is still informative as it suggests that these viruses do not pose any serious problems to apple growers. These growers can use this information to manage apple orchards accordingly.
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Details
- Title
- Effect of novel apple virus combinations on commonly used apple rootstocks and their association with apple decline in Washington state.
- Creators
- Alex Cross
- Contributors
- Scott Harper (Advisor)Hanu Pappu (Committee Member)Kiwamu Tanaka (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Plant Pathology, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 60
- Identifiers
- 99900652101901842
- Language
- English
- Resource Type
- Thesis