Thesis
Effects of carbon assimilation and light on growth and cell activity of Haematococcus pluvialis under mixotrophic conditions
Washington State University
Master of Science (MS), Washington State University
05/2016
Handle:
https://hdl.handle.net/2376/103371
Abstract
Microalgae are one of the most attractive resources for biofuel and various high-value products. Numerous studies have focused on mass cultivation and production of high-value products using microalgae for commercial and industrial applications. Microalgae also show potential for environmental protection due to their ability to absorb greenhouse gases. However, large-scale production of microalgae has encountered bottlenecks due to issues related to low biomass content, easy contamination and limited product accumulation. Biomass content is one of the most important factors for product accumulation and productivity. Until now, low biomass remains as a major problem in commercial algae production. Thus, this study aimed to increase the growth rate of microalgae to provide more biomass for producing high value co-products. First, the cultivation modes and environmental factors affecting the growth and product accumulation of Haematococcus pluvialis were investigated. The effect of organic carbon and light on its growth and biomass content was studied. The findings revealed that high growth could be obtained under the mixotrophic cultivation mode. The results also showed that a ribose of 1.45g/L was the optimal organic carbon source and concentration to increase growth and activate cell activity. After seven days, ribose of 41.4% was assimilated and 31% motile cells existed in ribose-culturing conditions compared with only 7% motile cells remaining, using sodium acetate as a carbon source. Ribose assimilation was promoted at high light intensity. However, cell activity was affected negatively by high light intensity, which induced cell inactivity and red stage transition. More active cell division and motile cells were obtained at lower light intensity, which prolonged algal division and growth at the green stage.
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Details
- Title
- Effects of carbon assimilation and light on growth and cell activity of Haematococcus pluvialis under mixotrophic conditions
- Creators
- Na Pang
- Contributors
- Shulin Chen (Chair)Birgitte Ahring (Committee Member) - Washington State University, Chemical Engineering and Bioengineering, School ofHaluk Resat (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Systems Engineering, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Number of pages
- 63
- Identifiers
- 99900525015601842
- Language
- English
- Resource Type
- Thesis