Thesis
Effects of polymer chemistry, concentration and pH on doxorubicin release kinetics from hydroxyapatite-PCL-PLGA composite for bone tissue engineering
Washington State University
Master of Science (MS), Washington State University
12/2015
Handle:
https://hdl.handle.net/2376/100527
Abstract
Incorporation of polymers into implant surface as carriers of drug can minimise chances of toxic effects in the body, and can also help deliver effective doses of drugs locally to the target tissues as compared to the oral drug delivery approach. This study aimed to understand the effects of the different compositions and concentration of polymeric matrix and pH of the release media towards the drug release kinetics. The coating characteristics and the drug along with their interactions with the polymer coating and the calcium phosphate matrix govern the release kinetics substantially. In this study, we report doxorubicin release behaviour from polycaprolactone (PCL) and poly-lactide-glycolide (PLGA) based hydroxyapatite (HAp). Doxorubicin is an anthracycline chemotherapeutic drug with a narrow therapeutic index. Three different compositions of the polymers with PCL, PLGA and a blend of PCL - PLGA with Hap in the implants are studied for doxorubicin release kinetics at pHs 7.4 and 5.0. Burst and uncontrolled release kinetics has been observed from bare HAp whereas, all the polymers incorporated implants showed sustained release kinetics. Percentages of the polymers are varied to investigate the effects of the polymer chemistry on the release kinetics. PCL incorporated samples were able to control the release more than the PLGA or blend incorporated samples. The hydrophilic-hydrophobic and hydrophobichydrophobic interaction between the polymer and the drug depending on the pHs are believed to be a pivotal factor controlling the release kinetics from the samples in contrast to the degradation or the dissolution process. No cytotoxicity was observed due to the drug incorporation at the early time points when cultured with human osteoblast cells. This study mainly bases on the comprehension of the effects of chemistry, environment and polymer - drug interactions towards the design of drug delivery devices.
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Details
- Title
- Effects of polymer chemistry, concentration and pH on doxorubicin release kinetics from hydroxyapatite-PCL-PLGA composite for bone tissue engineering
- Creators
- Dishary Banerjee
- Contributors
- Susmita Bose (Chair)Amit Bandyopadhyay (Committee Member) - Washington State University, Mechanical and Materials Engineering, School ofAnita Vasavada (Committee Member) - Washington State University, Chemical Engineering and Bioengineering, School of
- Awarding Institution
- Washington State University
- Academic Unit
- Mechanical and Materials Engineering, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Number of pages
- 99
- Identifiers
- 99900525096801842
- Language
- English
- Resource Type
- Thesis