Thesis
Electrospray ion mobility-time of flight- mass spectrometry for the detection of inorganic anions and proteins in aqueous media
Washington State University
Master of Science (MS), Washington State University
2007
Handle:
https://hdl.handle.net/2376/105317
Abstract
Electrospray ionization - atmospheric pressure - ion mobility - time of flight - mass spectrometry is used as a tool to investigate: A) [Chapters 2 &3] The likelihood of using a stand alone IMS for field analysis of inorganic anionic environmental contaminants and/or biotoxins, represented in this case by small proteins of similar mass, found in aqueous media - i.e. drinking water, storm water runoff, lakes, rivers, etc... This was achieved by relating reduced mobility values to various mass identified analytes of interest as well as looking for baseline resolution of both individual anions and proteins as well as synthetic mixtures that presumably mimic what might be seen in a real world sample. In addition two actual samples, taken in Seatlle, WA, are analyzed for the presence of anionic environmental contaminants of interest using the ESI-AP-IMtofMS instrument. B) [Chapter 3] The application of protein charge state trend lines, as identified in an ESIAP-IMtofMS experiment, to the identification of intact proteins in mixtures, as might be done in the field of proteomics, is investigated. Some success with rapid identification of proteins in a mixture, based on said trend lines, is realized.
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Details
- Title
- Electrospray ion mobility-time of flight- mass spectrometry for the detection of inorganic anions and proteins in aqueous media
- Creators
- Steven John Klopsch
- Contributors
- H. H. Hill (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemistry, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525377801842
- Language
- English
- Resource Type
- Thesis