Thesis
Epidemiology of the resistance to Wheat streak mosaic virus in perennial wheat
Washington State University
Master of Science (MS), Washington State University
2006
Handle:
https://hdl.handle.net/2376/103346
Abstract
Perennial wheat has anticipated benefits as an alternative cropping system in eastern Washington, including reduced planting costs, improved wildlife habitat, reduced soil erosion and increased soil quality. Wheat streak mosaic virus (WSMV) is widespread and common in this region of the US. Though epidemics are rare in annual winter wheat, this research demonstrates that WSMV is a serious threat to perennial wheat since it persists through successive years of regrowth. Thirty-three perennial lines derived from Thinopyrum sp. were evaluated for WSMV resistance in a growth chamber. Lines 03JP011, 03JP031 and 03JP039 had reduced virus replication, as indicated by lower ELISA values and less severe symptoms, compared to a susceptible control. Lines 03JP031 and 03JP039 were tested for temperature-sensitive resistance at 25°C, but reactions did not differ from those at 22°C. Progeny of 03JP031 and 03JP039 were evaluated for segregation of resistance, and inheritance is complex. In field plots, six perennial lines, including 03JP039, and three annual wheat controls were planted at three locations for two consecutive years in eastern Washington. In first year perennial growth, WSMV was detected in 16% of rows of line 03JP039 by ELISA, but only 5% were symptomatic, as compared with susceptible annual wheat controls in which 65 to 94% of rows were symptomatic. In second year perennial growth, 56% of 03JP039 rows developed symptoms and WSMV was detectable, and 100% of the five other perennial lines were symptomatic. Variable symptoms between first and second year growth may be explained by temperature sensitivity, susceptibility of initial perennial regrowth to expression of WSM symptoms, and/or heterogeneity within perennial wheat lines. WSMV natural infection was studied in bordering plots in two seasons; symptoms were observed in three perennial lines, and was not detected by ELISA in line 03JP039, nor were symptoms apparent. Compared with susceptible controls, perennial wheat had reduced virus replication in both controlled environment and field plots, especially line 03JP039. These results demonstrate that resistance to WSMV is present in perennial wheat and should be useful to plant breeders.
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Details
- Title
- Epidemiology of the resistance to Wheat streak mosaic virus in perennial wheat
- Creators
- Leigh Ann Harrison
- Contributors
- Timothy D. Murray (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Plant Pathology, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525178601842
- Language
- English
- Resource Type
- Thesis