Thesis
Evaluation of the biliary and brain distribution of technetium -99M-sestamibi in ABCB1 normal dogs before and after treatment with spinosad
Washington State University
Master of Science (MS), Washington State University
2012
Handle:
https://hdl.handle.net/2376/103210
Abstract
Objective—To determine whether the reported drug-drug interaction between the flea medication spinosad and ivermectin is attributable to inhibition of Pglycoprotein by spinosad. Animals—6 healthy adult dogs with the ABCB1 wildtype genotype. Procedures—The study was conducted as a prospective, masked, randomized crossover design. Six dogs were allocated to 2 groups; each dog served as its own control animal. Dogs in one of the groups received spinosad at the manufacturer’s recommended dose; the other group received no treatment. Forty-eight hours later, scintigraphic imaging of the head and abdomen were performed with the 99mTc- radiolabeled P-glycoprotein substrate methoxy-isobutyl-isonitrile (sestamibi) in both groups of dogs. After a washout period of 60 days, the dogs in each group received the alternate treatment, and scintigraphic imaging again was performed 48 hours later. Gallbladder-to-liver and brain-to-neck musculature ratios of 99mTcsestamibi were calculated for each dog and compared between treatments. Results—No significant difference in gallbladder-to-liver or brain-to-neck musculature ratios were found between treatments. Conclusions and Clinical Relevance—Results provided evidence that spinosad did not inhibit P-glycoprotein function 48 hours after spinosad was administered at the manufacturer’s recommended dose. Further investigations will be necessary to elucidate the mechanism of the reported toxic interaction between spinosad and ivermectin.
Metrics
3 File views/ downloads
7 Record Views
Details
- Title
- Evaluation of the biliary and brain distribution of technetium -99M-sestamibi in ABCB1 normal dogs before and after treatment with spinosad
- Creators
- Christopher S. Mackay
- Contributors
- Katrina L. Mealey (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525379901842
- Language
- English
- Resource Type
- Thesis