Thesis
Evaluation of urinary levels of free 8-isoprostane 2[alpha] in healthy dogs
Washington State University
Master of Science (MS), Washington State University
08/2020
DOI:
https://doi.org/10.7273/000004168
Handle:
https://hdl.handle.net/2376/125075
Abstract
The incurable condition known as ascending-descending myelomalacia (ADM) that occurs in dogs with severe spinal cord injury has a poorly understood pathophysiology. In this condition, it is suspected that normal self-regulatory mechanisms fail, and the changes incurred by the spinal cord can move high enough to a level to damage the nerves innervating respiratory muscles leading to respiratory paralysis and death. There is no known effective treatment and patients are humanely euthanized. No ante mortem diagnostics exist to detect ADM to allow for intervention of novel therapies early on in the disease process. Dysregulated oxidative stress is suspected to play a role in the development of ADM and naturally occurring biomarkers measuring oxidative stress have been investigated in veterinary medicine. Isoprostanes are biomarkers shown to be reliable and elevated in vivo in many forms of oxidative stress in various human conditions, and urinary levels of 8-isoprostane 2[alpha] have been found to be significantly elevated in in dogs that developed ADM showing promise for possible ante mortem diagnosis of ADM. Urinary levels of free 8-isoprostane 2[alpha] levels have been measured in dogs with spinal cord injury; however, a normal reference interval of urinary levels of free 8-isoprostane 2[alpha] levels has not yet been established in an appropriate number of healthy, non-spinal cord injured dogs. The specific aim of the current study was to establish a reference interval for urinary levels of free 8-isoprostane 2[alpha] in healthy dogs using a commercially available ELISA kit that has been previously validated in dogs. Commercially available competitive enzyme immunoassay (ELISA), isoprostane purification, and urinary creatinine assay kits were used to measure urinary levels of both 8-isoprostane 2[alpha] and creatinine. Isoprostane levels were normalized to creatinine levels to establish a reference interval for 8-isoprostane 2[alpha] in normal dogs. The mean urinary 8-isoprostane 2[alpha] normalized to the urinary creatinine levels are 99.3 ± 73.3 pg/mg with a reference interval of 13.6 (8.7, 20.5) to 294.4 (223.6, 371,0) pg/mg where the 90% confidence intervals are provided within the parentheses.
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Details
- Title
- Evaluation of urinary levels of free 8-isoprostane 2[alpha] in healthy dogs
- Creators
- Jessica Ashley Chavera
- Contributors
- Annie Vivian Chen-Allen (Advisor) - Washington State University, Veterinary Clinical Sciences, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900890769501842
- Language
- English
- Resource Type
- Thesis