Thesis
Extrusion-Based 3D-Printed Quercetin Loaded Polycaprolactone – Tricalcium Phosphate Scaffold for Bone Tissue Engineering Applications
Washington State University
Master of Science (MS), Washington State University
2023
DOI:
https://doi.org/10.7273/000006386
Abstract
The repair of critical-sized bone defects poses a significant challenge in advanced healthcare due to the limited regenerative capacity of bone tissue. Biomaterials offer a solution to this challenge; however, the complex interplay of numerous overlapping variables hinders the development of multifunctional biocomposites. Phytochemicals show promise in promoting bone growth, but their dose-dependent nature and physicochemical properties hinder their clinical use. To develop a comprehensive solution, a 3D-printed extrusion-based tricalcium phosphate-polycaprolactone (TCP-PCL) scaffold is augmented with quercetin and potassium chloride (KCl). This composite material demonstrates a compressive strength of 30 MPa showing promising stability for low load-bearing applications. The release of quercetin from the scaffold follows a biphasic pattern that persists for up to 35 days, driven via diffusion-mediated kinetics. The incorporation of KCl allows for tunable degradation rates of the scaffold and prevents initial rapid release. Functionalization of the scaffold facilitates the attachment and proliferation of human fetal osteoblasts (hfOB), resulting in a 2.1-fold increase in cell viability. Treated scaffolds exhibit a 3-fold reduction in osteosarcoma (MG-63) cell viability as compared to the untreated substrate. Ruptured cell morphology and decreased mitochondrial membrane potential indicate the antitumorigenic potential for treated scaffolds. Scaffolds with quercetin and quercetin-KCl (Q-KCl) demonstrate 76% and 89% reduction in bacterial colonies of Staphylococcus aureus, respectively. This study provides valuable insights into a promising strategy for bone tissue engineering in orthopedic repair.
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Details
- Title
- Extrusion-Based 3D-Printed Quercetin Loaded Polycaprolactone – Tricalcium Phosphate Scaffold for Bone Tissue Engineering Applications
- Creators
- Connor A Toulou
- Contributors
- Susmita Bose (Advisor)Amit Bandyopadhyay (Committee Member)Roland Chen (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- School of Engineering and Applied Sciences (TRIC)
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 79
- Identifiers
- 99901087839701842
- Language
- English
- Resource Type
- Thesis