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CC BY V4.0, Open Access
Thesis
GOOSEBERRY EXTRACT ENCAPSULATED LIPOSOMES ON PLASMA-ASSISTED HYDROXYAPATITE-COATED TI6AL4V FOR LOAD-BEARING APPLICATIONS
Washington State University
Master of Science (MS), Washington State University
12/2024
DOI:
https://doi.org/10.7273/000007239
Abstract
Bone defects such as fractures and osteoarthritis are becoming increasingly common, necessitating advancements in bone scaffold and implant research to create better patient outcomes. Phytochemicals found in natural sources show significant potential in promoting bone healing while combatting osteosarcoma and bacterial infections. Indian gooseberry (Phyllanthus emblica) (IG) has garnered attention due to its antioxidant, anti-inflammatory, chemopreventive, and antibacterial properties. Many of these benefits stem from the fruit’s high concentration of vitamin C (ascorbic acid). Unfortunately, the clinical applications of this compound have been limited due to its hydrophilicity, which causes rapid release. There is a significant knowledge gap regarding extending the release of hydrophilic substances and protecting sensitive substances from degradation caused by the surrounding environment. This work aimed to encapsulate IG within stable liposomes to control and sustain its release, thereby extending its beneficial properties. We hypothesize that encapsulation will reduce the burst release of IG and extend its release over time without compromising its beneficial properties. Liposomal encapsulation
provided 20% and 28% reductions in burst release within the first 24 hours in pH 5.0 and 7.4, respectively. This formulation loaded onto hydroxyapatite plasma-coated Ti6Al4V scaffolds reduced osteosarcoma viability by 2.4-fold after 11 days and Pseudomonas aeruginosa viability by 78% in 48 hours. This system of IG-loaded scaffolds demonstrates a promising strategy for promoting healing for high-load-bearing sites through stabilizing a volatile compound.
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Details
- Title
- GOOSEBERRY EXTRACT ENCAPSULATED LIPOSOMES ON PLASMA-ASSISTED HYDROXYAPATITE-COATED TI6AL4V FOR LOAD-BEARING APPLICATIONS
- Creators
- Bryson White
- Contributors
- Susmita Bose (Chair)Amit Bandyopadhyay (Committee Member)Kaiyan Qiu (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- School of Mechanical and Materials Engineering
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 77
- Identifiers
- 99901195539401842
- Language
- English
- Resource Type
- Thesis