Thesis
Gene Expression Analysis of Archived Canine Soft Tissue Sarcomas
Washington State University
Master of Science (MS), Washington State University
2022
DOI:
https://doi.org/10.7273/000005054
Abstract
Soft tissue sarcomas are a family of mesenchymal tumors common in dogs. Local invasion of this tumor type is often extensive, making complete surgical excision with curative intent difficult. Adjuvant therapy such as radiation therapy and chemotherapy is often required to address post-operative microscopic disease. Substantial genomic heterogeneity exists for both human and animal tumors, and the need for identification of better prognosticating gene expression patterns persists in a trend toward individualized medicine. Previous studies have examined a variety of genetic changes as potential drivers of tumorigenesis and tumor progression in soft tissue sarcomas, as well as attempts to differentiate between the different subtypes of soft tissue sarcomas; however, the use of next generation sequencing approaches have been scarcely reported previously in canine soft tissue sarcomas. As reported here, gene expression patterns in canine soft tissue sarcomas were examined using RNA-seq analysis of archived formalin-fixed and paraffin-embedded tumor samples. Functional overrepresentation analysis of differentially expressed genes highlighted differences between canine soft tissue sarcoma subtypes and comparative similarity between canine and human soft tissue sarcomas, identified involvement of common and tumor-type specific gene regulatory mechanisms such as those associated with tumorigenesis and transcription factors, as well as suggested potential therapy targets for future investigation.
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Details
- Title
- Gene Expression Analysis of Archived Canine Soft Tissue Sarcomas
- Creators
- Tien Tien
- Contributors
- Janean Fidel (Advisor)Eric Shelden (Committee Member)Rance Sellon (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 46
- Identifiers
- 99901019640401842
- Language
- English
- Resource Type
- Thesis