Thesis
Germination protocol for three cool-season native grasses: Elymus elymoides subspecies brevifolius "subspecies C" (SSP C), Elymus macrourus (ELMA7), and Elymus wawawaiensis (ELWA2)
Washington State University
Master of Science (MS), Washington State University
05/2016
Handle:
https://hdl.handle.net/2376/102325
Abstract
SSP C, ELMA7, and ELWA2 are available for sale in the United States by Pure Live Seed units, but are not listed in the Association of Official Seed Analysts (AOSA) Rules for Testing Seeds. The objective of this study was to develop standardized germination protocols for these three cool-season native grasses. The experiment was a split-plot, nested design with temperature as the main plot and accession and seed source as subplots for evaluating germination. Constant temperatures of 15oC, 20oC, and 25oC were the main parameters. ANOVA (P< 0.05) was done with SAS 9.2 and determined no statistical difference for the number of abnormal seedlings (P = 0.78) or dead seeds (P = 0.68) for all species and temperatures. SSP C had the highest germination at 15oC and 20oC. ELMA7 had no significant variation among all three temperature treatments. There was no significant difference in number of normal seedlings produced by ELWA2 at 15oC and 20oC. A refereed study with constant 20oC temperature and counts at 7, 14, and 21 d was conducted. Based upon the data from both studies, a germination temperature of 20oC for all three species was proposed as the test parameter. SSP C and ELWA2 had germinations, both as individuals and pooled samples, which were similar enough to indicate there was no need to extend testing to 21 d. Therefore, 14 d was proposed for the final count for SSP C and ELWA2. Analysis suggested a 21 d final count was necessary for ELMA7. A new rule proposal for each species was submitted 15 Oct. 2015 for review at the 2016 AOSA/SCST Joint Annual Meeting in Portland, OR with a goal for acceptance into the next AOSA Rules for Testing Seeds publication.
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Details
- Title
- Germination protocol for three cool-season native grasses
- Creators
- Paula M. Moore
- Contributors
- Richard T. Koenig (Chair)Vicki L. Bradley (Committee Member)William J. Johnston (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Crop and Soil Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Number of pages
- 99
- Identifiers
- 99900525074901842
- Language
- English
- Resource Type
- Thesis