Thesis
Glucosamine reduces glycogen storage in L6 skeletal muscle cells
Washington State University
Master of Science (MS), Washington State University
2007
Handle:
https://hdl.handle.net/2376/103226
Abstract
The impact of glucosamine supplementation on glycogen content in skeletal muscle fibers was examined in vitro using L6 myotubes. Serum starved L6 myotubes were exposed to either 5 mM or 25 mM glucose, with and without insulin (0 nM or 100 nM) and glucosamine (0 mM, 0.06 mM, 1 mM, 5 mM) for 1, 3 or 5 hr in culture. Glycogen content in L6 myotubes was quantified by image analysis following PAS staining. Insulin increased glycogen content in L6 myotubes across all treatments (P = 0.023). After exposure to a euglycemic condition, glycogen content was significantly increased at 3 and 5 hr as compared with glycogen content at 1 hr (P = 0.00002 and P = 0.006 respectively), independent of glucosamine treatment. Glucosamine supplementation in a hyperglycemic condition (25 mM glucose) reduced glycogen content in L6 myotubes. After 1 hr. incubation with 0.06 mM, 1 mM or 5 mM glucosamine supplementation, glycogen content was significantly less than that present in the absence of glucosamine (all P = 0.000 at each comparison, respectively). Additionally, glycogen content in L6 cells decreased over time in the 25 mM glucose treatment groups. Glycogen content was significantly reduced following exposure to 1 mM glucosamine versus results obtained at 3 and 5 hr (P = 0.000 and P = 0.001, respectively). These data indicate that glucosamine supplementation can reduce glycogen content in L6 myotubes, and this effect is dependent on glucose concentration in the media and the duration of exposure in culture.
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Details
- Title
- Glucosamine reduces glycogen storage in L6 skeletal muscle cells
- Creators
- Nicole Munoz
- Contributors
- Sally E. Blank (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Nutrition and Exercise Physiology, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525084501842
- Language
- English
- Resource Type
- Thesis