Thesis
Grape seed extract supplementation improves colonic cell differentiation via inhibiting WNT/Beta-Catenin pathway in IL-10-deficient mice
Washington State University
Master of Science (MS), Washington State University
2014
Handle:
https://hdl.handle.net/2376/103271
Abstract
Intestinal epithelium forms a stout barrier for absorbing and processing nutrients. It also involves in secreting compounds that influence microbial colonization, sensing both beneficial and harmful microbes, and modulation of immune responses. The intestinal epithelium barrier is regulated by various factors and signaling pathways. Changes in the rate of cell proliferation and differentiation impair the renewal of the intestinal epithelium that underlies many digestive diseases. Interleukin (IL)-10 plays a key role in the immunity of gastrointestinal tract. IL-10-deficient mice have been widely used as a model for studying intestinal damage. The Wnt/beta-catenin signaling is a crucial pathway in regulating cell proliferation and cell fate determination. Grape seed extract (GSE) is a mixture of v polyphenols extracted from grape seed and skin, which is known for its beneficial effects. In addition, GSE has been reported to reduce cell proliferation rate and induce cell differentiation in the epithelium of different animal models. The objective of this study was to explore the effect of GSE supplementation on colonic cell differentiation and proliferation, and whether these effects are regulated via the Wnt/beta-catenin pathway in IL-10-deficient mice. Results showed that GSE supplementation increased the colonic goblet cell numbers associated with increased MUC-2 and MUC-3 mRNA expression, and decreased the colonic proliferating cell nuclear antigen (PCNA) positive cells. Consistently, GSE supplementation decreased beta-catenin accumulation in colon crypts and the nuclei. These were associated with decreased phosphorylation of extracellular signal-regulated kinase (ERK), which is a key mediator regulating cell proliferation. Moreover, GSE supplementation also reduced the mRNA expression of two down-stream effectors of Wnt/beta-catenin signaling, c-Myc and cyclin D1. In summary, this study shows that GSE supplementation has the beneficial effects on gut epithelial health by improving colonic cell differentiation possibly via inhibiting the Wnt/beta-catenin pathway in IL-10-deficient mice.
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Details
- Title
- Grape seed extract supplementation improves colonic cell differentiation via inhibiting WNT/Beta-Catenin pathway in IL-10-deficient mice
- Creators
- Guan Yang
- Contributors
- Meijun Zhu (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Food Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525160201842
- Language
- English
- Resource Type
- Thesis