Thesis
HIGH-PROTEIN MODIFIED TRANSUDATE ABDOMINAL EFFUSION IN DOGS: A PROSPECTIVE COMPARATIVE PILOT STUDY OF THE DIAGNOSTIC POTENTIAL OF EFFUSION PROTEIN ELECTROPHORESIS
Washington State University
Master of Science (MS), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000003339
Handle:
https://hdl.handle.net/2376/124983
Abstract
In dogs, high-protein modified transudates (HPMT) are primarily a result of post-hepatic disease, such as right-sided congestive heart failure or pericardial disease, lesions obstructing blood flow from the hepatic veins or caudal vena cava into the right side of the heart or rarely post-sinusoidal diseases within the liver itself. This type of effusion is attributed to increased hydrostatic pressure within the liver sinusoids and has a relatively high-protein concentration believed to reflect contributions of protein-rich hepatic lymph. From this unique pathophysiologic mechanism, the assumption can be made that the protein in this type of abdominal effusion could have a particular distribution that influences the pattern of a protein electrophoretogram. In this prospective comparative pilot study, we hypothesized that the protein electrophoretogram of a HPMT abdominal effusion would have its own unique pattern compared to other types of abdominal effusion. A total of nineteen abdominal effusion samples were analyzed by electrophoresis. Electrophoretograms from HPMT and exudate were compared to determine if there was a predictable electrophoretogram pattern that characterized post-hepatic HPMT. A statistically significant difference was identified in the absolute concentration of the total protein (p=0.045), of the albumin (p=0.015) and the proportion of albumin (p=0.047). These values were higher in the HPMT group than the exudate group. Statistically significant differences were also identified in the proportion of alpha2 (p=0.014) and beta globulins (p= 0.035). These values were higher in the exudate group than the HPMT group. When removing cases with presumed intra-abdominal disease from the HPMT group, a significant difference was only seen in the absolute concentration of albumin (p=0.01) and the proportion of albumin (p=0.033) and alpha2 globulins (p=0.031). The absolute concentration and proportion of albumin were still higher in the HPMT group than the exudate group and the proportion of alpha2 globulins was also still higher in the exudate group than the HPMT group. In conclusion, these results can give the clinician some guidance on the possible type of fluid but make it difficult to predict that a type of fluid such as a HPMT can be diagnosed solely through an electrophoresis analysis.
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Details
- Title
- HIGH-PROTEIN MODIFIED TRANSUDATE ABDOMINAL EFFUSION IN DOGS: A PROSPECTIVE COMPARATIVE PILOT STUDY OF THE DIAGNOSTIC POTENTIAL OF EFFUSION PROTEIN ELECTROPHORESIS
- Creators
- Antonella Reber
- Contributors
- Rance K Sellon (Advisor)Jillian M Haines (Committee Member)Cleverson Desouza (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 34
- Identifiers
- 99900652102501842
- Language
- English
- Resource Type
- Thesis