Thesis
Identification of loci associated with susceptibility to Mycobacterium avium subspecies Paratuberculosis tissue infection in cattle
Washington State University
Master of Science (MS), Washington State University
2015
Handle:
https://hdl.handle.net/2376/100194
Abstract
Johne's disease (JD) is an infectious and incurable bacterial disease caused by Mycobacterium avium subspecies paratuberculosis (Map). With no effective vaccine, little progress has been made in reducing disease prevalence through control programs based on reducing cattle's exposure to Map. An alternative approach to limiting the prevalence of JD is to breed cattle with an increased resistance to Map tissue infection. Therefore, the objective of this study was to identify loci associated with Map tissue infection as the first step in selecting cattle with an increased resistance to Map to reduce the prevalence of JD. Holstein cattle from the Northeast (NE) (127 controls and 92 Map tissue infected cases), the Pacific Northwest (PNW) (75 cases and 130 controls), and Jersey cattle (16 cases and 41 controls) were genotyped using the Illumina Bovine SNP50 BeadChip. A genome wide association analysis (GWAA) was conducted with PNW, Jersey, and NE+PNW (combined) populations using the Efficient Mixed-Model Association eXpedited - genomic relationship matrix statistical approach. Associations with JD were tested for allelic, additive, dominant and recessive models using the Wellcome Trust Consortium guidelines. Nine quantitative trait loci (QTL) were identified in the PNW, twelve QTL were identified in the Jerseys, and six QTL were identified in the NE+PNW cattle. The identification of QTL associated with Map tissue infection provides tools to develop predicted transmitting abilities (PTA) for selecting cattle more resistant to JD. The QTL identified in this study can be incorporated into commercial genotyping panels used for computing PTAs and selecting breeding stock more resistant to JD. By selecting cattle with more resistance to JD, this will elicit a permanent reduction in the prevalence of this fatal and incurable disease.
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Details
- Title
- Identification of loci associated with susceptibility to Mycobacterium avium subspecies Paratuberculosis tissue infection in cattle
- Creators
- Jennifer Nicole Kiser
- Contributors
- Holly L. Neibergs (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Animal Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900524808001842
- Language
- English
- Resource Type
- Thesis