Thesis
Improvement of Fourier transform ion cyclotron resonance mass spectrometry detection technology
Washington State University
Master of Science (MS), Washington State University
2010
Handle:
https://hdl.handle.net/2376/105595
Abstract
Described herein are two novel FTICR-MS cells designed to increase performance: Trapping Ring Electrode Cell (TREC) and excite-coupled Trapping Ring Electrode Cell(eTREC). TREC permits the ability to maintain coherent ion motion at larger initial excited cyclotron radii by decreasing the change in radial electric field with respect to z-axis position in the cell. Resolving power approaching the theoretical limit was achieved using the novel TREC technology; over 420,000 resolving power was observed on melittin [M+4H]4+ species when employed under modest magnetic field strength (3T). A tenfold gain in signal-to-noise ratio (S/N) is demonstrated over the closed cylindrical cell optimized with common potentials on all ring electrodes. The observed frequency drift during signal acquisition over long time periods was also significantly reduced, resulting in improved resolving power. eTREC technology provides greater linearity in the excitation electric field along with minimized variation in radial trapping field during detection. Using this technology sensitivity was increased by >50%, resolution of 13C2 and 34S fine structure peaks was achieved with the peptide MMMMG (~330,000 RP) on a 3 Tesla system, and the limit of detection was significantly reduced.
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Details
- Title
- Improvement of Fourier transform ion cyclotron resonance mass spectrometry detection technology
- Creators
- Chad R. Weisbrod
- Contributors
- James E. Bruce (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemistry, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525394601842
- Language
- English
- Resource Type
- Thesis