Thesis
Inactivation of Cronobacter (Enterobacter) sakazakii using different antimicrobial agents and the effect of sanitizers on biofilm formation properties
Washington State University
Master of Science (MS), Washington State University
2009
Handle:
https://hdl.handle.net/2376/104286
Abstract
Enterobacter sakazakii is an opportunistic pathogen that represents a health risk to neonates. It is a known contaminant of infant formula milk (IFM) and has been associated with cases of necrotizing enterocolitis and infant meningitis. Many studies have been performed on the growth conditions and inactivation of the microorganism. Control of this microbe is important since it affects highly susceptible populations. The purpose of this study was to investigate the inactivation of E. sakazakii (five strains) inoculated into IFM using different antimicrobials, and to evaluate the effectiveness of different disinfectants for the removal of biofilms on plastic surfaces. Results showed that out of all the antimicrobials used, the combination of lactic acid (0.2% v/v) and copper (50 ppm) proved to be the most effective, where the individual use of copper at 50 ppm and lactic acid at 0.2% v/v did not have a significant effect on the growth of E. sakazakii (P > 0.05), but the combination of both agents produced a significant inhibition of growth in both rehydrated infant formula milk (RIFM) and powdered infant formula milk (PIFM) after 2 hours (P < 0.05). The effectiveness of three different household dishwashing liquids, and a sodium hypochlorite solution in the removal of biofilms formed on plastic surfaces was also studied, and it was found that quaternary ammonium compounds are the most effective, and that treatment time plays an important role in the elimination of the biofilms, since higher reductions in population were observed as the treatment times increased.
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Details
- Title
- Inactivation of Cronobacter (Enterobacter) sakazakii using different antimicrobial agents and the effect of sanitizers on biofilm formation properties
- Creators
- Luis Fernando Castro
- Contributors
- Barbara Rasco (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Food Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525004401842
- Language
- English
- Resource Type
- Thesis