Thesis
Lithium-doped tricalcium phosphate and tricalcium phosphate/polycaprolactone particulate composite for bone-graft and drug-delivery applications
Washington State University
Master of Science (MS), Washington State University
2011
Handle:
https://hdl.handle.net/2376/100247
Abstract
The first part of this study investigated the influence of Li-doping on the physical, mechanical and biological properties of b-Tricalcium phosphate (b-TCP) ceramic. b-TCP was synthesized with 0.22 mol % Li and 0.44 mol % Li using a wet coprecipitation method. A second set of samples was prepared by physically-mixing 0.10 mol% Li and 0.18 mol% Li with commercial b-TCP using a ball-milling method. In comparison to the undoped samples, the 0.44 mol% composition exhibited a decrease in compressive strength of 58.3 MPa, whereas the 0.22 mol% showed no statistically significant change. Compared to the undoped samples, the 0.10 mol% and 0.18 mol% samples showed a decrease of 63.6 and 50.9 MPa in compressive strength, respectively. Immunohistochemistry and MTT assay results showed an increase in the hFOB differentiation and proliferation activities with the addition of Li-dopant. Proliferation of hFOB cells from day 3 to day 7 showed an increase of 64% in the 0.22 mol% Li-TCP, compared to 30% in the undoped synthesized samples. Similarly, the physically-mixed samples showed an increase of 76% for the 0.10 mol% samples compared to 51% in the undoped commercial samples. The higher level of Li-doping decreased the proliferative activity in both groups, indicating that Li-doping is only beneficial at the lower concentrations. Immunohistochemistry and confocal microscopy results also showed that the levels of Li-doping in b-TCP can enhance the hFOB cellular differentiation compared to undoped b-TCP. Our results indicate that a low amount of Li-doping has the potential for applications in bone graft materials. The second part of this work investigated the effects of surface area, as well as surface composition on the loading and release behavior of bovine serum albumin (BSA). To begin, the BSA was adsorbed onto bare TCP powder of various surface areas, as well as onto a Tricalcium phosphate/polycaprolactone (TCP/PCL) particulate composite, which were constructed from the various bare TCP powders. It was concluded that surface area has a significant effect on the loading and release of BSA, whereby the amount of BSA adsorbed and released is increased as surface area is increased. It was also determined that PCL had no significant effect on the loading and release behavior of BSA
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Details
- Title
- Lithium-doped tricalcium phosphate and tricalcium phosphate/polycaprolactone particulate composite for bone-graft and drug-delivery applications
- Creators
- Joseph Matthew Edgington
- Contributors
- Susmita Bose (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Mechanical and Materials Engineering, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525169501842
- Language
- English
- Resource Type
- Thesis