Thesis
Lovastatin decreases TGF- 1 concentration of glomerular endothelial cells cultured in high glucose
Washington State University
Master of Science (MS), Washington State University
2008
Handle:
https://hdl.handle.net/2376/100775
Abstract
Background: Diabetic nephropathy (DN) is the leading cause of morbidity, mortality and end stage renal disease in patients with diabetes mellitus.1, 2 Glomerular fibrosis is a key pathological process in DN and is enhanced in diabetes. Endothelial cells cultured under high glucose conditions can enhance the production of transforming growth factor beta (TGF-β1). HMG-CoA reductase inhibitors (statins) have been shown to decrease markers of fibrosis in cell culture models of diabetes.1-3 Lovastatin (LOV) decreases TGF-β production in mesangial cells and various types of endothelial cells cultured in high glucose conditions.1-8 However, to date, there are no studies reporting the effects of LOV on the production of TGF-β by rat glomerular endothelial cells (RGEC) cultured under high glucose conditions. The purpose of this study was to investigate the effects of LOV on the production of TGF-β in a diabetic cell culture model using RGEC. We hypothesize that LOV will inhibit the increases in total TGF-β concentration that typically occur in high glucose models. Methods: RGEC were grown to confluence in normal glucose (5.5 mM) media supplemented with 10% FBS. Once confluent, RGEC were exposed to high glucose (25 mM) media alone or with 10 μM LOV, and incubated for 48 h. Media samples were taken at 12, 24 and 48 h and frozen (-80° C) with protease inhibitor cocktail and subsequently assayed for total TGF-β1. Results: Total TGF-β1 protein produced by RGEC exposed to 10 μM LOV was significantly lower than groups exposed to high glucose alone at 12 h (p=0.017) and 24 h (p=0.020). We conclude that 10 μM LOV was effective at decreasing total TGF-β1 concentration in RGEC exposed to high glucose media.
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Details
- Title
- Lovastatin decreases TGF- 1 concentration of glomerular endothelial cells cultured in high glucose
- Creators
- Erinn Claire Lanxon-Cookson
- Contributors
- Emily Carolyn Johnson (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Pharmacy and Pharmaceutical Sciences, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525182901842
- Language
- English
- Resource Type
- Thesis