Thesis
Metabolic consequence in arogenate dehydratase-modified Arabidopsis and Populus
Washington State University
Master of Science (MS), Washington State University
2013
Handle:
https://hdl.handle.net/2376/100138
Abstract
Arogenate dehydratase (ADT) is responsible for generation of the aromatic amino acid phenylalanine in higher plants. The impact of modulating ADT gene expression on lignin composition has previously been studied in Arabidopsis with single/multiple knockouts (KO) of its 6-membered gene family. This established that various permutations of knockouts of the encoded ADT genes resulted in differentially reduced lignin contents. In the current study, levels of the most abundant phenylpropanoid/phenylpropanoid-acetate derived low molecular weight metabolites were assessed via UPLC-UV-MS in stem, leaf, and root tissues of various Arabidopsis ADT mutants and wild-type, as well as in young and older leaf tissues of hybrid white poplar (Populus tremula x Populus alba). For Arabidopsis, the data obtained for these metabolites was then compared and contrasted with the differentially reduced lignin contents obtained in the various transgenic lines, with the hypothesis being that pathway related v metabolite abundance would correlate well with same. Through a combination of UV and MS data processed using statistical software (xcms, metaXCMS and CAMERA), the levels of the six most abundant phenylpropanoid/phenylpropanpoid acetate metabolites (quercetin and kaempferol glycosides, sinapoyl malate) were also differentially reduced in lignin-deficient lines of Arabidopsis during growth and development. In Populus, using the same statistical approach, reductions in levels of various phenylpropanoid/phenylpropanoid-acetate derived and shikimatechorismate pathway metabolites were accompanied by concomitant increases in amounts of presumed upstream pathway salicylates.
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Details
- Title
- Metabolic consequence in arogenate dehydratase-modified Arabidopsis and Populus
- Creators
- Alan D. Budgeon
- Contributors
- Norman G. Lewis (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemistry, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900524884801842
- Language
- English
- Resource Type
- Thesis