Thesis
Molecular mapping of the V locus in pea (Pisum sativum L.)
Washington State University
Master of Science (MS), Washington State University
2019
Handle:
https://hdl.handle.net/2376/101460
Abstract
Peas are a nutritious annual cool season legume crop are widely consumed as a vegetable. Garden peas, in which the immature seed is eaten as a vegetable are used as either fresh, canned, or frozen product. Edible pod peas, eaten at a very immature stage, either fresh or frozen, are growing in popularity. Edible pod peas have either reduced or no parchment in the inner pod wall. Parchment is governed by two non-allelic genes - P and V. When at least one copy of the dominant allele of each gene is present, pods have parchment. If one locus is homozygous recessive and the other has a dominant allele, the parchment is reduced and if both loci are homozygous recessive, parchment is absent. The objectives of this study were to 1) determine the genotype of Apollo; and 2) identify SNPs associated with parchment using a recombinant inbred line (RIL) population derived from pea cv 'Aragorn' (with parchment) and cv 'Apollo' (with reduced parchment). In this study, Apollo was crossed to two tester lines with known genotypes and based on the phenotype of the F1 pods was determined to be PPvv. The F6-derived RIL population was phenotyped in controlled conditions and genotyped using the two-enzyme genotyping-by sequencing (GBS) approach. Of the 172 members of the RIL population, 91lines had reduced parchment), and 81 lines had strong parchment. Using GBS, 2507 high-quality SNPs were identified and a linkage map was constructed using a total of 2757 markers. The linkage map identified seven linkage groups and had a total length of 1063 cM with an average density of 2.5 markers per cM. In the results of these analyses using the RIL population, Apollo was determined to be PPvv and V was localized at 173.16 cM on chromosome 5 (LG III). The nearest SNPs to V are at 168.3 cM and 176.9 cM. The identity of the genotype of Apollo can be confirmed by crossing Apollo and the two tester lines with other lines with known genotypes.
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Details
- Title
- Molecular mapping of the V locus in pea (Pisum sativum L.)
- Creators
- Derya Eroglu Karaca
- Contributors
- Rebecca J. McGee (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Crop and Soil Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525373901842
- Language
- English
- Resource Type
- Thesis