Thesis
Monoclonal Antibody Reagents for Use in Clinical Flow Cytometry and Research in Dogs
Washington State University
Master of Science (MS), Washington State University
01/2022
DOI:
https://doi.org/10.7273/000004503
Handle:
https://hdl.handle.net/2376/124838
Abstract
Since the convening of the first international workshop in 1993 to compare the specificity of monoclonal antibodies (mAb) developed by different laboratories for use in clinical flow cytometry and research in dogs, further efforts to develop additional mAbs have been carried out by individual laboratories. As reported here, we have continued to use two approaches to identify mAbs for use in dogs. The first approach was initiated during participation in international workshops focused on identification of mAbs that recognize epitopes conserved on orthologues of molecules in multiple veterinary species. The second approach has been to immunize mice with peripheral blood leukocytes from different species, alone or mixed to increase chances for generating mAbs to conserved epitopes. For dogs, an effort was made to immunize mice with leukocytes present in blood and also, a B cell lymphoma to obtain mAbs reactive with leukocyte differentiation molecules (LDM) useful in clinical flow cytometry and research. As described here both approaches have yielded additional mAbs for use in dogs.
Metrics
3 File views/ downloads
21 Record Views
Details
- Title
- Monoclonal Antibody Reagents for Use in Clinical Flow Cytometry and Research in Dogs
- Creators
- David Kim
- Contributors
- Cleverson deSouza (Advisor)William Davis (Committee Member)Janean Fidel (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 33
- Identifiers
- 99900882331001842
- Language
- English
- Resource Type
- Thesis