Thesis
Mutations in ovis aries TMEM154 are associated with reduced ovine lantivirus proviral concentration in one sheep flock
Washington State University
Master of Science (MS), Washington State University
2013
Handle:
https://hdl.handle.net/2376/101764
Abstract
Ovine lentivirus (OvLV), also called ovine progressive pneumonia virus or maedi-visna, is present in 24% of U.S. sheep. Like human immunodeficiency virus, OvLV is a macrophagetropic lentivirus that causes lifelong infection. The production impacts from OvLV are due to a range of disease symptoms, including pneumonia, arthritis, mastitis, body condition wasting, and encephalitis. There is no cure and no effective vaccine for preventing OvLV infection. However, breed differences in prevalence and proviral concentration indicate a genetic basis for susceptibility to OvLV. Animals with high blood proviral concentration show increased tissue lesion severity, so proviral concentration represents a live animal test for control post-infection in terms of proviral replication and disease severity. Recently, it was found that sheep with two copies of TMEM154 haplotype 1 (encoding lysine at position 35) had lower odds of OvLV infection. In the present study, we examined the relationship between OvLV control postinfection and variants in two genes, TMEM154 and CCR5, in 4 flocks containing 2260 sheep. We found two copies of TMEM154 haplotype 1 were associated with reduced OvLV proviral concentration in one flock (P< 0.02), which identified the same favorable diplotype for control post-infection as for odds of infection. However, the frequencies of undesirable TMEM154 alleles were too low in the other 3 flocks to test. The CCR5 promoter deletion did not have consistent association with OvLV proviral concentration. Future work in flocks with more balanced allele frequencies is needed to confirm or refute TMEM154 association with control of OvLV post-infection.
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Details
- Title
- Mutations in ovis aries TMEM154 are associated with reduced ovine lantivirus proviral concentration in one sheep flock
- Creators
- Fahad Alshanbari
- Contributors
- Stephen N. White (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525176801842
- Language
- English
- Resource Type
- Thesis