Thesis
Myostatin attenuation In vivo reduces adiposity, but activates adipogenesis
Washington State University
Master of Science (MS), Washington State University
2015
Handle:
https://hdl.handle.net/2376/100996
Abstract
A potentially novel approach for treating obesity includes attenuating myostatin as this increases muscle mass and decreases fat mass. Notwithstanding, conflicting studies report that myostatin stimulates or inhibits adipogenesis and it is unknown whether reduced adiposity with myostatin attenuation results from changes in fat deposition or adipogenesis. We therefore quantified changes in the stem, transit amplifying and progenitor (STP) cell pool in white and brown adipose tissue (WAT & BAT) using label-retaining wild-type and mstn-/- (Jekyll) mice. Muscle mass was larger in adult Jekyll mice and that of WAT and BAT was smaller while label induction was equal in all tissues from wild-type and Jekyll mice. The number of label retaining cells (LRCs), however, dissipated quicker in both WAT and BAT of Jekyll mice as it was only 25% and 17%, respectively, of wild-type cell counts 1 month after induction. Adipose cell density was significantly higher in Jekyll mice, particularly in BAT as the density increased over time concomitant with LRC disappearance, consistent with enhanced expansion and differentiation of the STP pool in Jekyll mice. The stromal vascular cells from Jekyll WAT and BAT differentiated into mature adipocytes and at a faster rate than wild-type cells. Jekyll WAT cells also proliferated quicker and responded better to insulin like growth factor (IGF1) stimulation. However, BAT cells proliferated at approximately the same rate as wild-type. These results suggest that myostatin attenuation enhances adipogenesis and that the reduced adiposity in mstn-/- animals. Pharmacological targeting of myostatin would likely diminish fat deposition and/or nutrient partitioning away from fat stores and may be a useful tool in the reduction of obesity.
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Details
- Title
- Myostatin attenuation In vivo reduces adiposity, but activates adipogenesis
- Creators
- Naisi Li
- Contributors
- Dan Rodgers (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Animal Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525153201842
- Language
- English
- Resource Type
- Thesis