Thesis
PLATELET ADDITIVE SOLUTION ALLOWS FOR PROLONGED STORAGE OF FUNCTIONAL CANINE PLATELET CONCENTRATE
Washington State University
Master of Science (MS), Washington State University
01/2022
DOI:
https://doi.org/10.7273/000004562
Handle:
https://hdl.handle.net/2376/125374
Abstract
Thrombocytopenia is a common disease seen in veterinary medicine that can result in life threatening bleeding making it a frequent indication for platelet transfusions. For this reason, a readily available supply of platelets is needed for transfusion into these critical patients. The objective was to assess storage lesion development, platelet function, and bacterial growth in canine platelet concentrates (PC) stored in a platelet additive solution (PAS) or a plasma control at 4℃ for 21 days. The study was a prospective, ex-vivo experimental controlled study at the Washington State University, Veterinary Teaching Hospital using ten units of canine platelet concentrates collected from the blood bank. The PC were separated into two bags, one containing 100% plasma and other 35% plasma and 65% of a PAS (Plasma-Lyte A) and stored at 4℃ for 21 days. At days 0, 7, 14 and 21, PC was analyzed for the presence of swirling, aggregate formation, platelet counts, platelet indices, glucose, lactate, lactate dehydrogenase, PvCO2, PvO2, aggregation via light aggregometry, activation percentages using flow cytometry, and bacterial growth. The results showed that cold stored PC in both PAS and plasma control maintained mean pH > 6.8 and mean lactate < 9.0 mmol/L over 21 days with no difference in glucose utilization. Swirl was maintained in both solutions for most days (76/80 combined total samples) with no difference in aggregate formation between solutions. The PvCO2 was higher in plasma on all days (p < 0.001) with no difference in PvO2. Platelet indices did not reflect significant storage lesion development in either solution. Lactate dehydrogenase did not differ between solutions but did increase from day 7 to 21. Mean maximal aggregation percentage was overall reduced but with no significant difference between solutions. The only observed difference in mean activation percentage between solutions was in PAS on day 7, which was significantly higher than plasma (p<0.05). No bacterial growth occurred during storage. In conclusion, cold storage in PAS and plasma allowed PC to be stored for up to 21 days with minimal storage lesion development, maintenance of platelet function, limited platelet activation, and no bacterial growth within stored bags.
Metrics
Details
- Title
- PLATELET ADDITIVE SOLUTION ALLOWS FOR PROLONGED STORAGE OF FUNCTIONAL CANINE PLATELET CONCENTRATE
- Creators
- Avin Arjoonsingh
- Contributors
- Jillian M Haines (Advisor)Jane Wardrop (Committee Member)Rance Sellon (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 38
- Identifiers
- OCLC#: 1371059169; 99900898640201842
- Language
- English
- Resource Type
- Thesis