Thesis
Paper-based cationic isotachophoretic platform for detection of disease markers
Washington State University
Master of Science (MS), Washington State University
05/2021
DOI:
https://doi.org/10.7273/000004278
Handle:
https://hdl.handle.net/2376/125267
Abstract
The objective of this study is two-fold: to develop a method to electrophoretically concentrate positively charged gold nanoparticles (GNPs) using paper-based cationic isotachophoresis (ITP), as well as explore an approach for modifying negatively charged GNPs with avidin to produce conditions favorable to cationic ITP on the paper-based platform. The rationale is that ITP can be integrated into lateral flow assay technology to enhance the sensitivity of the analytical technology. This work describes and demonstrates important factors to consider when designing an ITP system including the selection and modification of the reaction media, and the design of the ITP discontinuous buffer system including the selection of the leading and terminating ions, counterions, pH, and ionic strength of the system. Furthermore, this work demonstrates the effectiveness of using avidin to alter the electrophoretic mobility of biotinylated GNPs by reversing the negative surface charge to favor cationic ITP conditions. This study demonstrates that the techniques described have potential to enhance the effectiveness of paper-based point-of-care technology and bring this this technology closer to practical applications.
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Details
- Title
- Paper-based cationic isotachophoretic platform for detection of disease markers
- Creators
- Devon Michael McCornack
- Contributors
- Wenji Dong (Advisor) - Washington State University, School of Chemical Engineering and Bioengineering
- Awarding Institution
- Washington State University
- Academic Unit
- Voiland College of Engineering and Architecture
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900896413501842
- Language
- English
- Resource Type
- Thesis