Thesis
Passive immunotherapy using egg-yolk antibodies to reduce Campylobacter jejuni from the intestinal tract of broiler chickens
Washington State University
Master of Science (MS), Washington State University
2011
Handle:
https://hdl.handle.net/2376/102322
Abstract
The aim of this study was to produce chicken egg-yolk derived immunoglobulins (IgY) against five C. jejuni colonization associated proteins (CAPs) and evaluate their efficacy to reduce cecal colonization by C. jejuni in experimentally infected chickens. In this study the recombinant C. jejuni CAPs including CadF, CmeC, FlaA, FlpA and major outer membrane protein (MOMP) were expressed in the E. coli expression host and purified by cobalt-column affinity chromatography method. Recombinant CAPs were then used as antigens to hyperimmunize laying hens to induce production of anti-C. jejuni CAP-specific immunoglobulins (IgY). All immunized hens produced anti-C. jejuni CAP-specific antibody responses . Anti-C. jejuni CAP-specific IgY levels were significantly higher in serum samples and egg-yolks obtained from immunized hens as compared with the non-immunized hens. Egg-yolks collected from immunized and non-immunized hens were lyophilized to obtain egg-yolk powder (EYP) with or without anti-C. jejuni CAP-specific IgY. Three-day old chickens were challenged with C. jejuni strain F38011 (108 CFU/bird). At 9 days post-infection (i.e. 12 days of age), hyperimmunized egg-yolk powder (HEYP) against each C. jejuni CAP, nonimmunized egg-yolk powder (NEYP), and a cocktail containing equal parts of five HEYP were included in feed at 10% concentration (w/w). Treated feed was provided ad libitum for three days starting from 12 days until the end of the experiment at day fifteen. Positive control and negative control group was given standard feed. Cecal samples were collected at the end of experiment to enumerate C. jejuni counts. However, incorporation of IgY in the form of lyophilized egg-yolk powder in feed had no significant effect (P =0.47) on levels of cecal colonization by C. jejuni when compared with infection positive control.
Metrics
6 File views/ downloads
9 Record Views
Details
- Title
- Passive immunotherapy using egg-yolk antibodies to reduce Campylobacter jejuni from the intestinal tract of broiler chickens
- Creators
- Salma R. Al-Adwani
- Contributors
- Devendra H. Shah (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525124901842
- Language
- English
- Resource Type
- Thesis